Study on the Intervention Effect and Mechanism on Insulin Resistant Mice of Total Flavone from Melastoma dodecandrum Lour.

This experiment was conducted to explore the intervention effect and mechanism on insulin resistance (IR) mice of total flavonoids from Melastoma dodecandrum Lour.(TFMD).The model of IR mice was established by intragastric administration of high-fat emulsion,while 600,300 and 150 mg/kg TFMD were administered once daily for 30 days.After the end of the experiment the mices' fasting blood glucose (FBG),fasting serum insulin (FINS),serum total cholesterol(TC),triglyceride (TG),high density lipoprotein(HDL) were determined.The mRNA expression level of liver insulin receptor(InsR),fat peroxisome proliferator-activated receptor-γ(PPAR-γ),and glucose transporter 4 gene (GLUT4) in skeletal muscle were detected by Real-time quantative PCR,and the protein levels were detected by immunohistoche mical method.The results showed that TDMF could reduce the body weight of IR mice,decrease the level of serum FBG,FINS and HOMA-IR,increase the level of ISI,decrease the content of serum TG,TC and LDL,and increase the content of HDL(P<0.01,P<0.05);And the mRNA expression of INSR,PPAR-γ in liver and GLUT4 in skeletal muscle of IR mice were increased(P<0.01,P<0.05),and the protein expression level of InsR,PPAR-γ in liver and GLUT4 in skeletal muscle of IR mice were increased(P<0.01,P<0.05).The results confirmed that the TFMD could relieve experimental insulin resistance in mice,and the activity was related to the regulation of glucose and lipid metabolism and the enhancement of insulin sensitivity.