Inheritance of resistance to angular leaf spot (Pseudomonas syringae pv. lachrymans) in cucumber and identification of molecular markers linked to resistance |
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Authors: | H. Olczak-Woltman,G. Bartoszewski,W. M dry, K. Niemirowicz-Szczytt |
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Affiliation: | Department of Plant Genetics, Breeding and Biotechnology, Warsaw University of Life Sciences, ul. Nowoursynowska 159, 02-776 Warsaw;and;Department of Biometry, Warsaw University of Life Sciences, ul. Nowoursynowska 159, 02-776 Warsaw, Poland |
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Abstract: | Angular leaf spot is a common disease of cucumber ( Cucumis sativus ) caused by Pseudomonas syringae pv. lachrymans . Genetics of resistance to this disease was investigated using two sets of parameters: (i) disease severity, i.e. the number and size of necrotic and chlorotic lesions on the infected leaves, and (ii) presence or absence of a chlorotic halo around the necrotic spots on the infected leaves. Disease severity appears to be controlled by multiple genes and the heritability of the resistance was estimated to be 53%. The presence or absence of the chlorotic halo was determined to be governed by a single gene, with the presence of the halo (the susceptible phenotype) being a dominant character. A RAPD marker linked to the gene conferring the chlorotic halo was identified. Genetic distance between this marker, OP-AO07, a polymorphic 420 bp amplicon in the DNA of the susceptible plants, and the locus encoding the chlorotic halo was estimated to be 13 cM. |
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Keywords: | Cucumis sativus disease resistance marker assisted selection |
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