| 咖啡叶片DNA提取方法的比较研究 |
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| 引用本文： | 黄丽芳,闫林,董云萍,范睿,王晓阳,庞永青.咖啡叶片DNA提取方法的比较研究[J].热带农业科学,2011,31(12):42-45. |
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| 作者姓名： | 黄丽芳 闫林 董云萍 范睿 王晓阳 庞永青 |
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| 作者单位： | 中国热带农业科学院香料饮料研究所／海南省热带香辛饮料作物遗传改良与品质调控重点实验室／农业部香辛饮料作物遗传资源利用重点实验室,海南万宁,571533 |
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| 基金项目： | 国家自然科学基金项目(No.31000740、No.31071475); 公益性行业(农业)科研专项经费项目(No.200903024); 南亚热作专项(No.11RZZY-08) |
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| 摘 要： | 以咖啡成熟叶片为材料，进行多种DNA提取方法比较研究，并通过琼脂糖凝胶电泳和紫外分光光度计检测所提的DNA样品。结果表明，CTAB改良Ⅰ和CTAB改良Ⅱ均可用于咖啡叶片的DNA提取，尤其是CTAB改良Ⅱ法，提取的DNA产量较CTAB改良Ⅰ高，能满足后续分子生物学研究的要求。
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| 关 键 词： | 咖啡 DNA提取 SDS—CTAB CTAB |
Comparison of the DNA Extraction Methods from Coffee Leaves |
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| HUANG Lifang YAN Lin DONG Yunping FAN Rui WANG Xiaoyang PANG Yongqing.Comparison of the DNA Extraction Methods from Coffee Leaves[J].Chinese Journal of Tropical Agriculture,2011,31(12):42-45. |
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| Authors: | HUANG Lifang YAN Lin DONG Yunping FAN Rui WANG Xiaoyang PANG Yongqing |
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| Institution: | HUANG Lifang YAN Lin DONG Yunping FAN Rui WANG Xiaoyang PANG Yongqing(Spice and Beverage Crops Research Institute/Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical and Beverage Crops/Ministry of Agriculture Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops,CATAS,Wanning,Hainan 571533,China) |
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| Abstract: | 7 kinds of different methods for coffee DNA extraction on mature leaves of coffee were compared in the study. Then the purified DNA was detected through agarose gel electrophoresis and spectrophotometer, which indicated that modified CTAB Ⅰ and modified CTAB Ⅱ extraction methods were both suitable for extracting DNA of coffee, especially the method of modified CTAB Ⅱ obtained higher concentration DNA sample, which was available to the further molecular biology study. |
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| Keywords: | coffee(Coffea canephora) DNA extraction SDS-CTAB CTAB |
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