| 苦瓜总皂甙含量测定及超声提取最佳条件的研究 |
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| 引用本文： | 邱红,魏佑营,王军伟,魏秉培,曹克友,吴静. 苦瓜总皂甙含量测定及超声提取最佳条件的研究[J]. 山东农业科学, 2008, 0(6) |
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| 作者姓名： | 邱红 魏佑营 王军伟 魏秉培 曹克友 吴静 |
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| 作者单位： | 山东农业大学园艺科学与工程学院/作物生物学国家重点实验室,山东,泰安,271018;山东农业大学园艺科学与工程学院/作物生物学国家重点实验室,山东,泰安,271018;山东农业大学园艺科学与工程学院/作物生物学国家重点实验室,山东,泰安,271018;山东农业大学园艺科学与工程学院/作物生物学国家重点实验室,山东,泰安,271018;山东农业大学园艺科学与工程学院/作物生物学国家重点实验室,山东,泰安,271018;山东农业大学园艺科学与工程学院/作物生物学国家重点实验室,山东,泰安,271018 |
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| 基金项目： | 泰安市大学生科技创新引导计划项目 |
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| 摘 要： | 建立了苦瓜总皂甙的超声提取和分光光度测定方法,并优化了超声提取的提取温度、乙醇浓度、料液比和提取时间等因素。结果显示,超声波提取法最佳工艺组合为:70%乙醇,液料比为30∶1,振荡频率为60%,70℃超声提取30 m in/次,重复提取2次;且分光光度测定法操作简便、快速、准确,可作为苦瓜制品提取及质量评价方法。
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| 关 键 词： | 苦瓜 总皂甙 超声提取 分光光度法 |
Spectrophotometric Determination of Total Saponins from Momordica charantia and Condition Optimization of Ultrasonic Extraction |
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| QIU Hong,WEI You-ying,WANG Jun-wei,WEI Bing-pei,CAO Ke-you,WU jing. Spectrophotometric Determination of Total Saponins from Momordica charantia and Condition Optimization of Ultrasonic Extraction[J]. Shandong Agricultural Sciences, 2008, 0(6) |
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| Authors: | QIU Hong WEI You-ying WANG Jun-wei WEI Bing-pei CAO Ke-you WU jing |
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| Abstract: | The methods for ultrasonic extraction and spectrophotometric determination of total saponins from Momordica charantia were established;the extracting temperature,alcohol content,the ratio of material to solvent,extracting time and so on were also optimized.The results showed that the optimum conditions for ultrasonic extraction were 30∶1 of 70% alcohol solution to Mormordica charantia weight,extraction for 2 times repeatedly and 30 minutes every once under 70℃ with 60% oscillation frequency.The method is simple,rapid and accurate and suitable for the quality evaluation of Momordica charantia material and products. |
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| Keywords: | Momordica charantia Total saponins Ultrasonic extraction Spectrophotometric method |
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