柳枝稷种子愈伤组织诱导及分化

高效再生体系是建立遗传转化体系的基础和前提，本研究以柳枝稷（Panicum virgatum）成熟种子为外植体，对柳枝稷再生体系的建立进行探索。使用70%乙醇及0.1% HgCl2采用两步法灭菌，以MS培养基为基本培养基，研究不同质量浓度2,4 D与6 BA组合对愈伤组织诱导的影响，不同质量浓度GA3对愈伤组织分化的影响。结果表明，70%乙醇处理20 s，然后用0.1% HgCl2处理5 min，种子活力保存最好，愈伤组织诱导率最高；愈伤组织诱导培养基添加5 mg·L-1 2,4 D与1.2 mg·L-1 6 BA为最佳组合，其诱导率最高，质量最好；添加0.5 mg·L-1GA3分化成苗的效果最好。最终得到柳枝稷最优的再生体系：MS培养基为基本培养基，添加5 mg·L-1 2,4 D及1.2 mg·L-1 6 BA进行愈伤组织诱导；添加0.5 mg·L-1 GA3分化、再生。

Callus induction and differentiation of switchgrass Panicum virgatum seeds

The efficient regeneration system is the basis and premise for a genetic transformation system.The mature seeds of switchgrass(Panicum virgatum) were used as the explant to establish the regeneration system of switchgrass.After sterilizing the seeds by 70% ethanol and 0.1% HgCl2 with two-step asepsis,MS culture medium was basic culture medium to determine the influence of different concentrations of 2,4-D and 6-BA on callus induction and the influence of GA3 on differentiation.The results of this study showed that the seeds activities of switchgrass were well preserve and the callus induction rate was the highest when seeds were treated by 70% ethanol for 20 s and then by 0.1% HgCl2 for 5 min.The quality of callus were better when the MS consisted of 5 mg·L-1 2,4-D and 1.2 mg·L-1 6-BA,and the differentiation and regeneration of callus was the best as 0.5 mg·L-1 GA3 were added into the MS.The best regeneration system of switchgrass included that MS was the basic medium,5 mg·L-1 2,4-D and 1.2 mg·L-1 6-BA was added to induct the callus,and 0.5 mg·L-1 GA3 was used to differentiate and regenerate.