| 热激因子基因AtHSFa1a提高烟草的耐热性研究 |
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| 引用本文： | 万发香,田婷婷,杜小兵,苏承刚,张兴国. 热激因子基因AtHSFa1a提高烟草的耐热性研究[J]. 西南大学学报(自然科学版), 2011, 33(10) |
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| 作者姓名： | 万发香 田婷婷 杜小兵 苏承刚 张兴国 |
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| 作者单位： | 南方山地园艺学教育部重点实验室西南大学园艺园林学院; |
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| 基金项目： | 重庆市重大攻关项目(CSTC,2010AA1019)资助 |
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| 摘 要： | 克隆了拟南芥热激因子AtHSFa1a,并由热激启动子AtHSP70b控制,构建了双元表达载体pV-70bAtHSFa1a,并经农杆菌介导转化烟草,获得了转基因植株,并检测了其耐热性指标.结果显示:与对照相比,转基因烟草的电导率较低,丙二醛含量增幅较小,SOD酶活性较高,初步表明其对高温具有更高的耐受性.
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| 关 键 词： | 烟草 拟南芥热激因子AtHSFa1a 耐热性 |
Heat Shock Transcription Factor Gene AtHSFa1a Can Enhance Thermotolerance of Tobacco |
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| WAN Fa-xiang,TIAN Ting-ting,DU Xiao-bing,SU Cheng-gang,ZHANG Xing-guo Kay Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education,School of Horticulture , L,scape Architecture,Southwest University,Chongqing ,China. Heat Shock Transcription Factor Gene AtHSFa1a Can Enhance Thermotolerance of Tobacco[J]. Journal of southwest university (Natural science edition), 2011, 33(10) |
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| Authors: | WAN Fa-xiang TIAN Ting-ting DU Xiao-bing SU Cheng-gang ZHANG Xing-guo Kay Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education School of Horticulture L scape Architecture Southwest University Chongqing China |
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| Affiliation: | WAN Fa-xiang,TIAN Ting-ting,DU Xiao-bing,SU Cheng-gang,ZHANG Xing-guo Kay Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education,School of Horticulture and Landscape Architecture,Southwest University,Chongqing 400716,China |
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