新牧一号苜蓿MvP5CS基因的克隆和功能分析

为研究苜蓿（Medicago sativa）耐盐分子机制,为抗逆分子育种提供依据,通过同源克隆和RT PCR技术克隆了新牧一号杂花苜蓿（M.varia Xinmu 1）的MvP5CS基因, MvP5CS全长2 148 bp,Genbank登录号为：EU371644。荧光定量PCR分析发现,在盐胁迫下MvP5CS基因表达明显上调,说明 MvP5CS与苜蓿的耐盐性相关。利用农杆菌介导的叶盘转化法,将MvP5CS基因成功转入烟草（Nicotiana tabacum）,盐胁迫下转MvP5CS基因T1代烟草萌发率和根长均高于非转基因烟草。表明新牧一号苜蓿P5CS基因能够提高转基因烟草的耐盐性。

Cloning and function analysis of MvP5CS in Medicago varia xinmu-1

The MvP5CS of Medicago varia Xinmu-1 was cloned by RT-PCR and homology cloning.The MvP5CS was 2 148 bp long and the accession number of Genbank was EU371644.A Real-time PCR assay showed that the level of MvP5CS transcriptions was obviously up-regulated in the seedlings after longer time salinity treatment,indicating that MvP5CS was related to salt tolerance.The MvP5CS genes of M.varia Xinmu-1 was transferred successfully into tobacco via agrobacterium mediation.The germination percentage and root length of T1-generation transgenic tobacco were higher than that of non-transgenic tobacco under salt stress,which indicated that the MvP5CS enhanced the salt tolerance of transgenic tobacco,and this provide references for salt-tolerance molecular mechanism and future stress-resistance molecular breeding of M.sativa.