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| 牛结核杆菌mpb-83基因的扩增和表达 | |
| 引用本文： | 李树春,斯肯达尔,杨宏军,何洪斌,王长法,杨少华,高运东,仲跻峰.牛结核杆菌mpb-83基因的扩增和表达[J].山东农业科学,2010(6):10-12. |
| 作者姓名： | 李树春 斯肯达尔 杨宏军 何洪斌 王长法 杨少华 高运东 仲跻峰 |
| 作者单位： | 1. 新疆农业大学,新疆,乌鲁木齐,830052;山东省农业科学院奶牛研究中心,山东,济南,250100 2. 新疆农业大学,新疆,乌鲁木齐,830052 3. 山东省农业科学院奶牛研究中心,山东,济南,250100 |
| 基金项目： | 山东省中青年科学家基金，山东省农业重大应用技术创新课题(2009)，现代农业产业技术体系项目 |
| 摘 要： | 采用PCR方法扩增牛结核杆菌mpb-83基因，并将其连接到T3克隆载体，然后克隆到原核表达载体PET-32a中，构建重组质粒PET-32a—mpb-83。以重组质粒转化大肠杆菌BL21，用NI柱纯化，最后纯化产物在SDS—PAGE中检测。结果得到约664bp的目的片段，且MPB-83可在大肠杆菌BL21中高效表达。 |
| 关 键 词： | 牛结核杆菌 mpb-83基因 克隆 原核表达 |
Cloning and Expression of mbp-83 Gene of Mycobacterium bovis |
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| LI Shu-chun,Skender,YANG Hong-jun,HE Hong-bin,WANG Chang-fa,YANG Shao-hua,GAO Yun-dong,ZHONG Qi-feng.Cloning and Expression of mbp-83 Gene of Mycobacterium bovis[J].Shandong Agricultural Sciences,2010(6):10-12. | |
| Authors: | LI Shu-chun Skender YANG Hong-jun HE Hong-bin WANG Chang-fa YANG Shao-hua GAO Yun-dong ZHONG Qi-feng |
| Institution: | LI Shu-chun1,2,Skender1,YANG Hong-jun2,HE Hong-bin2,WANG Chang-fa2,YANG Shao-hua2,GAO Yun-dong2,ZHONG Qi-feng2(1.Xinjiang Agricultural University,Urumqi,830052,China,2.Research Center of Cow,Sh,ong Academy of Agricultural Sciences,Jinan 250100,China) |
| Abstract: | The mpb-83 gene of Mycobacterium bovis was cloned by PCR method and was linked with the T3 vector.Then they were inserted into the prokaryotic expression vector PET-32a,which was expressed in E.coli BL21.The purification of mpb-83 was achieved by NI-NTA agarase and the purified product was analyzed by SDS-PAGE.The results showed that about 664 bp fragment was obtained,and the MPB-83 of Mycobacterium bovis was highly expressed in E.coli. |
| Keywords: | Mycobacterium bovis mbp-83 gene Cloning Prokaryotic expression |
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