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| 郏县红牛SREBP-1c基因84 bp缺失突变的检测及其对6个生长性状的影响 | |
| 引用本文： | 黄永震,王居强,马桂变,张恩平,淮永涛,马亮,雷初朝,牛晖,肖杰,陈宏. 郏县红牛SREBP-1c基因84 bp缺失突变的检测及其对6个生长性状的影响[J]. 畜牧兽医学报, 2010, 41(10) |
| 作者姓名： | 黄永震 王居强 马桂变 张恩平 淮永涛 马亮 雷初朝 牛晖 肖杰 陈宏 |
| 作者单位： | 1. 西北农林科技大学动物科技学院,陕西省农业分子生物学重点实验室,杨凌,712100 2. 河南省肉牛工程技术研究中心,郑州,450003 3. 河南省平顶山市畜牧局,平顶山,467000 |
| 基金项目： | 河南基础与前沿技术研究计划项目，国家"863"计划项目，国家自然科学基金，国家科技支撑计划，农业部肉牛产业体系项目，国家转基因专项子课题 |
| 摘 要： | 旨在研究牛SREBP-1c基因内含子7区域84bp缺失的遗传多态性及其对生长性状的影响。本试验以中国地方品种郏县红牛共计441头个体为实验动物,通过DNA测序技术和琼脂糖凝胶电泳方法检测该区域缺失突变的多态性。结果发现,在该突变位点检测到2种基因型:WW和WD型,等位基因W和D频率分别为0.8651和0.1349。He、Ne和PIC值都很低,说明在本突变位点遗传多态性不够丰富。该突变位点处于Hardy-Weinberg平衡状态(P0.05)。该位点的多态性与郏县红牛6个生长性状指标关联分析显示,郏县红牛WD基因型个体在12、18、24月龄的体质量和胸围显著高于WW基因型个体(P0.01或P0.05),基因型WD个体在18、24月龄的体斜长显著高于WW基因型个体(P0.05),基因型WD个体在18月龄的平均日增体质量显著高于WW基因型个体(P0.01)。初步认为WD型是提高黄牛体质量和体尺指标性状的有利基因型。本研究结果表明,黄牛SREBP-1c基因内含子7区域84bp缺失位点可作为郏县红牛生长性状的潜在分子育种标记,具有很大的研究价值。 |
| 关 键 词： | SREBP-1c基因 缺失突变 郏县红牛 生长性状 |
Deletion Mutation of the Bovine SREBP-1c Gene of Jiaxian Red Cattle and Its Affecting on Growth Traits |
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| HUANG Yong-zhen,WANG Ju-qiang,MA Gui-bian,ZHANG En-ping,HUAI Yong-tao,MA Liang,LEI Chu-zhao,NIU Hui,XIAO Jie,CHEN Hong. Deletion Mutation of the Bovine SREBP-1c Gene of Jiaxian Red Cattle and Its Affecting on Growth Traits[J]. Chinese Journal of Animal and Veterinary Sciences, 2010, 41(10) | |
| Authors: | HUANG Yong-zhen WANG Ju-qiang MA Gui-bian ZHANG En-ping HUAI Yong-tao MA Liang LEI Chu-zhao NIU Hui XIAO Jie CHEN Hong |
| Affiliation: | HUANG Yong-zhen 1,WANG Ju-qiang 2,MA Gui-bian 3,ZHANG En-ping 1,HUAI Yong-tao 1,MA Liang 1,LEI Chu-zhao 1,NIU Hui 2,XIAO Jie 2,CHEN Hong 1(1.Key Laboratory of Agricultural Molecular Biology of Shanxi,College of Animal Science and Technology,Northwest A&F University,Yangling 712100,China,2.Research Center of Cattle Engineering Technology of Henan,Zhengzhou 450003,3.Pingdingshan Animal Husbandry Bureau,Pingdingshan 467000,China) |
| Abstract: | The present study focused on screening the genetic variation within bovine SREBP-1c gene intron 7 and analyzing its effect on growth traits in 441 individuals belonging to Jiaxian Red cattle.In this study,the 84 bp deletion mutation of SREBP-1c gene was detected by using the DNA sequencing and agarose electrophoresis methods.The PCR products of SREBP-1c gene exhibited two genotypes WW and WD,and two alleles were revealed:W and D.Genotype WW was the predominant genotype and W was the predominant allele in th... |
| Keywords: | SREBP-1c gene deletion mutation Jiaxian Red cattle growth traits |
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