昆明小鼠不同性别早期胚胎体外培养发育潜力观察

自主设计2对引物,提取已知性别小鼠肝细胞DNA,扩增雄性小鼠特有的Sry基因及雌、雄小鼠共有的ZFX基因,建立双重PCR性别鉴定方法.提取小鼠早期8细胞胚胎单卵裂球、双卵裂球DNA,分别进行双重PCR性别鉴定,选择出早期8细胞胚胎的适宜模板量;将已测性别的8细胞胚胎按照雌、雄性别分开培养,观察统计雌、雄8细胞胚胎发育至囊胚的比率.试验结果显示,与单个卵裂球DNA模板量相比,双卵裂球的模板量检出率高,两者之间差异显著(75.00%Vs 93.33%,P<0.05);经过性别鉴定的雄性8细胞胚胎比雌性8细胞胚胎发育至囊胚的比率高,两者之间差异显著(53.33%Vs 33.33%,P<0.05).结果表明,采用双重PCR性别鉴定方法,以双卵裂球的DNA量为模板能够有效的对小鼠早期8细胞胚胎进行性别鉴定;雄性胚胎在体外培养条件下比雌性胚胎具有更好的发育潜力.

Developmental potentiality of different sex early embryos of kunming mouse in vitro

In order to establish a double PCR for sex identification,we designed two pairs of primers to amplify mouse Sry gene sequence and DFX gene from mouse liver cell.The first sequence was only male mouse had,the other was both male and female mouse had.With double PCR,we selected proper number of template from single blastomere and double blastomere.We separated male and female embryos which had been identificated to culture in vitro.We found that there was a significant contrast between double blastomeres and single blastomere(75.00% Vs 93.33%,P<0.05).Male 8-cell embryos had a better developmental rate of blastula than those of female and there was a significant contrast between them(53.33% Vs 33.33%,P<0.05).The results indicated that with double PCR and double blastomeres template,we could success to do sex identification on 8-cell embryos of mouse,and the male embryos had a better developmental potentiality than female in vitro.