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| 猪流行性腹泻病毒(PEDV)M蛋白原核表达载体的构建 | |
| 引用本文： | 高慎阳,刘延庆,李一经.猪流行性腹泻病毒(PEDV)M蛋白原核表达载体的构建[J].中国兽医学报,2009,29(12). |
| 作者姓名： | 高慎阳 刘延庆 李一经 |
| 作者单位： | 1. 辽宁医学院,畜牧兽医学院,辽宁,锦州,121001;东北农业大学,动物医学院,黑龙江,哈尔滨,150030 2. 黑龙江省讷河市畜牧水产局,黑龙江,讷河,161300 3. 东北农业大学,动物医学院,黑龙江,哈尔滨,150030 |
| 摘 要： | 为消除M蛋白抑茵现象使其得到稳定表达,本试验采用两种策略:一是将M基因截短得到编码M蛋白膜外区的基因序列(M'),构建重组表达载体pGEX-6p-M';二是将M基因改造,在其N端和C端同时加上GST基因,即构建pGEX-6p-M-GST重组表达栽体. |
| 关 键 词： | PEDV M蛋白 GST 原核表达栽体 |
The construction of prokaryotic expression vector of PEDV membrane protein |
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| GAO Shen-yang,LIU Yan-qing,LI Yi-jing.The construction of prokaryotic expression vector of PEDV membrane protein[J].Chinese Journal of Veterinary Science,2009,29(12). | |
| Authors: | GAO Shen-yang LIU Yan-qing LI Yi-jing |
| Abstract: | To obtain its stable expression,we took tWO strategies to eliminate the antibacterial phenomenon of membrane protein'in this work.First,the truncated fragment of PEDV M gene which encodes the exterior of the viral envelope(M')was subcloned into pGEX-6p-1 for the construction of pGEX-6p-M'.Second,in the same way the recombinant plasmids pGEX-6p-M-GST were constructed with GST genes linking at N-terminal and C-terminal of M gene. |
| Keywords: | PEDV membrane protein GST prokaryotic expression vector |
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