鸡组织中尼卡巴嗪残留HPLC检测方法的研究

建立了尼卡巴嗪标识残留物在鸡组织中残留的HPLC检测方法.样品用乙腈提取,正己烷脱脂,C18固相小柱净化.以乙腈:水(52: 48)为流动相,紫外检测器在波长340 nm处检测.在该检测条件下,尼卡巴嗪标示残留物DNC的检测限为31.25μg/kg,定量限为100μg/kg.DNC工作液在31.25～8 000 μg/kg范围内药物浓度与峰面积呈良好的线性关系(r=0.999 9).尼卡巴嗪在鸡肌肉、肝脏和肾脏中添加浓度分别为0.1、0.2和0.4 μg/g时,样品回收率在80.06%～89.87%之间.批内变异系数小于7.09%,批间变异系数小于6.34%.

Determination of nicarbazin residues in chicken tissues by high-performance liquid chromatography

A method was developed for the determination of nicarbazin in chicken tissues by high-performanceliquid chromatography.Nicarbazin was extracted from tissue samples with acetonitrile;and cleared up by C18.Nicarbazin was determined by HPLC in a single analysis on NH_2 column with Uv detection at 340 nm,and mobile phase was acetonitrile-water(52/48).The limits of detection was 31.25 μg/kg.The average recoveries of nicarbazin spiked to tissues were in range of 80.06% to 89.87% when fortified with 0.1,0.2 and 0.4 μg/g.The response for nicarbazin was linear in the range of 31.25-8 000 μg/kg(r=0.999 9,n-5).The within-day coefficient of variation(CV) and between-day CV were less than 7.09% and 6.34% respectively.The method can meet the demand of residue determination of nicarbazin in chicken tissues.