| 氟喹诺酮类药物残留免疫学检测方法的研究 |
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| 引用本文： | 魏东,杨正涛,李颖,郭昌明,刘辉,张乃生.氟喹诺酮类药物残留免疫学检测方法的研究[J].中国兽医学报,2009,29(12). |
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| 作者姓名： | 魏东 杨正涛 李颖 郭昌明 刘辉 张乃生 |
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| 作者单位： | 1. 吉林大学,畜牧兽医学院,吉林,长春,130062;河北北方学院,河北,张家口,075000
2. 吉林大学,畜牧兽医学院,吉林,长春,130062 |
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| 摘 要： | 以沙拉沙星(SALX)为半抗原,牛血清白蛋白(BSA)偶联后免疫兔,获得对多种氟喹酮类(FQNS)药物有特异性的广谱性抗体.以SALX与卵清白蛋白(OVA)的偶联物作包被原,建立并优化了间接竞争ELISA检测方法,对SALX最低检出限达19.319 μg/L,且对诺氟沙星、环丙沙星和恩诺沙星均有特异性识别,最低检测限分别是22.646、26.181和19.054 μg/L.结果表明,该抗体可用于多种氟喹诺酮类药物残留筛选检测.
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| 关 键 词： | 氟喹诺酮 残留 检测 酶免疫分析方法 |
Immunoassay method of detecting the residues of fluoroquinolones |
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| WEN Dong,YANG Zheng-tao,LI Ying,GUO Chang-ming,LIU Hui,ZHANG Nai-sheng.Immunoassay method of detecting the residues of fluoroquinolones[J].Chinese Journal of Veterinary Science,2009,29(12). |
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| Authors: | WEN Dong YANG Zheng-tao LI Ying GUO Chang-ming LIU Hui ZHANG Nai-sheng |
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| Abstract: | Sarafloxacin(SALX)was used as hapten coupling with bovine serum albumin to generate broad specifieity antibody against fluoroquinolones(FQNS).Assay performance was improved by use of a coating antigen,which conjuagated between the SALX and carrier protein(OVA).Typical detection limits for SALX was 19.319 μg/L.Different cross-reactions with the closely related FQNS,including NFLX,CPLX and EFLX,were detected with the detection limits of 22.646,26.181 and 19.054 μg/L,respectively.The result indicated that the antibody developed in this study was practicable to screen the residues of FQNS. |
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| Keywords: | fluoroquinolones(FQNS) residue detection ELISA |
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