| 农杆菌介导的植酸酶基因转化棉花的研究 |
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| 引用本文： | 郭彩菊,王省芬,张桂寅,迟吉娜,吴立强,李志坤,马峙英.农杆菌介导的植酸酶基因转化棉花的研究[J].中国农学通报,2009,25(20):68-71. |
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| 作者姓名： | 郭彩菊 王省芬 张桂寅 迟吉娜 吴立强 李志坤 马峙英 |
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| 作者单位： | 河北省作物种质资源重点实验室/河北农业大学,保定,071001 |
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| 基金项目： | 转基因生物新品种培育重大专项，河北省科技支撑计划重大项目，河北省自然科学基金重点项目 |
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| 摘 要： | 摘要：本研究采用农杆菌介导的方法，将外源植酸酶(Phy)基因导入棉花品种的胚性愈伤组织中，经培养获得再生植株。PCR检测证明植酸酶基因已经插入到棉花基因组中，晋棉14和陕724的转化效率分别为33%和8.3%。此外，本研究还对影响农杆菌侵染棉花胚性愈伤转化效率的因素，如卡那霉素浓度、胚性愈伤组织生长状态、受体材料的基因型等进行了较为系统的比较研究，旨在建立一种转化效率高、稳定性好的棉花农杆菌遗传转化体系。
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| 关 键 词： | 瓷玫瑰 瓷玫瑰 切花 保鲜 水分平衡 |
| 收稿时间： | 2009-05-22 |
| 修稿时间： | 2009-06-10 |
The Agrobacterium-Mediated PhyA Genetic Transformation for Embryogenic Calli of Cotton |
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| Guo Caiju,Wang Xingfen,Zhang Guiyin,Chi Jina,Wu Liqiang,Li Zhikun,Ma Zhiying.The Agrobacterium-Mediated PhyA Genetic Transformation for Embryogenic Calli of Cotton[J].Chinese Agricultural Science Bulletin,2009,25(20):68-71. |
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| Authors: | Guo Caiju Wang Xingfen Zhang Guiyin Chi Jina Wu Liqiang Li Zhikun Ma Zhiying |
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| Institution: | (Key Laboratory of Crop Germplasm Resources of Hebei/ Agricultural University of Hebei, Baoding 071001 ) |
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| Abstract: | Abstract: In this study, some transgenic cotton plants with a phytase gene were obtained successfully via agrobacterium-mediated method of embryogenic calli. Transformation efficiency of resistant plants about Jimian14 and Shann724 was 33% and 8.3% respectively. Effect of kanamycin concentration, the growth status of embryogenic calli and the genotype of the target material on the transformation efficicency had been assayed. The factors influencing agrobacterium-mediated cotton calli transformation were studied systematically in order to establish a highly efficient and stable genetic transformation system of cotton. |
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| Keywords: | cotton Agrobactetium-mediated phyA gene embryogenic calli genetic transformation |
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