| 玉米褪绿斑驳病毒实时荧光RT-PCR检测方法研究 |
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| 引用本文： | 闻伟刚,张建成,崔俊霞,张颖. 玉米褪绿斑驳病毒实时荧光RT-PCR检测方法研究[J]. 植物检疫, 2011, 0(1) |
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| 作者姓名： | 闻伟刚 张建成 崔俊霞 张颖 |
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| 作者单位： | 宁波出入境检验检疫局; |
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| 基金项目： | 浙江省分析测试项目(2009F70036); 质检公益性项目(201010256) |
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| 摘 要： | 玉米褪绿斑驳病毒(Maize chlorotic mottle virus,MCMV)是我国对外公布的检疫性有害生物。本研究根据该病毒外壳蛋白基因的保守序列,设计得到特异性引物及Taqman荧光探针,建立了MCMV的实时荧光RT-PCR方法,并对其灵敏度与特异性进行了研究。该方法针对2个不同来源的毒株均能得到典型扩增曲线,而没有从小麦线条花叶病毒、玉米粗缩病毒和玉米矮花叶病毒的RNA得到扩增曲线,表明引物与荧光探针具有良好的特异性。针对玉米褪绿斑驳病毒RNA不同稀释度样品,实时荧光RT-PCR检测低限达到10-5稀释度,检测灵敏度要比普通RT-PCR高出100倍。因此,本研究建立的MCMV实时荧光方法具有特异性强、灵敏度高和快速有效的优点。
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| 关 键 词： | 玉米褪绿斑驳病毒 实时荧光RT-PCR 检测 |
Detection of Maize chlorotic mottle virus by real-time fluorescent RT-PCR |
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| Wen Weigang,Zhang Jiancheng,Cui Junxia,Zhang Ying. Detection of Maize chlorotic mottle virus by real-time fluorescent RT-PCR[J]. Plant Quarantine, 2011, 0(1) |
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| Authors: | Wen Weigang Zhang Jiancheng Cui Junxia Zhang Ying |
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| Affiliation: | Wen Weigang,Zhang Jiancheng,Cui Junxia,Zhang Ying(Ningbo Entry-exit Inspection and Quarantine Bureau,Ningbo 315012,China) |
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| Abstract: | Maize chlorotic mottle virus(MCMV) is a quarantine pests issued by Chinese government.In this study,accord to the conservative sequences of the coat protein genes of different MCMV isolates,a real-time fluorescent RT-PCR method was established based on specific primers and TaqMan probe.Specific studies have shown that for the two MCMV strains from different sources,a typical amplification curve can be obtained,and not for the Wheat streak mosaic virus,Maize rough dwarf virus and Maize dwarf mosaic virus.Sen... |
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| Keywords: | Maize chlorotic mottle virus real-time fluorescent RT-PCR detection |
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