| 泡桐丛枝植原体染色体全长及两个rRNA操纵子定位研究 |
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| 引用本文： | 杨毅,杨旭光,林彩丽,田国忠,赵文军,李志红,朱水芳. 泡桐丛枝植原体染色体全长及两个rRNA操纵子定位研究[J]. 植物检疫, 2011, 0(4) |
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| 作者姓名： | 杨毅 杨旭光 林彩丽 田国忠 赵文军 李志红 朱水芳 |
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| 作者单位： | 中国农业大学农学与生物技术学院;中国检验检疫科学研究院动植物检疫研究所;中国林业科学研究院森林生态环境与保护研究所; |
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| 基金项目： | 质检公益性行业科研专项(200810517) |
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| 摘 要： | 本实验以泡桐丛枝植原体为材料,运用差速离心和脉冲场电泳(Pulsed Field Gel Electrophoresis,PFGE),获得了无寄主DNA污染、完整的泡桐丛枝植原体染色体,并用Southern blot杂交进行了验证,建立了植原体全基因组的研究方法。同时对泡桐丛枝植原体染色体进行内切酶I-CeuI不完全酶切,测定了染色体全长,并定位了2个非连锁的rRNA操纵子在染色体上的位置,完成了泡桐丛枝植原体部分物理图谱的构建。实验结果表明,泡桐丛枝植原体染色体全长约为1 100kb,2个rRNA操纵子在染色体上相距500～600kb。
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| 关 键 词： | 泡桐丛枝植原体 染色体 rRNA操纵子 脉冲场电泳 |
The chromosome size and the locations of the two rRNA operons of paulownia witches'-broom phytoplasma |
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| Yang Yi,,Yang Xuguang,Lin Caili,Tian Guozhong,Zhao Wenjun,Li Zhihong,Zhu Shuifang. The chromosome size and the locations of the two rRNA operons of paulownia witches'-broom phytoplasma[J]. Plant Quarantine, 2011, 0(4) |
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| Authors: | Yang Yi Yang Xuguang Lin Caili Tian Guozhong Zhao Wenjun Li Zhihong Zhu Shuifang |
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| Affiliation: | Yang Yi1,2,Yang Xuguang1,Lin Caili3,Tian Guozhong3,Zhao Wenjun2,Li Zhihong1*,Zhu Shuifang2(1.College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China,2.Institute of Animal and Plant Quarantine,Chinese Academy of Inspection and Quarantine,3.Research Institute of Forest Ecology,Environment and Protection,CAF) |
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| Abstract: | We established a method for research of the paulownia witches'-broom phytoplasma(PaWB) genome using differential centrifugation and pulsed field gel electrophoresis(PFGE).And the method confirmed by Southern blot.Partial digestions with I-CeuI and PFGE were used to determine the size of the PaWB chromosome,which was estimated to be about 1100kb.And we also located the two unlinked rRNA operons which were 500~600kb apart on PaWB chromosome. |
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| Keywords: | Paulownia witches'-broom phytoplasma chromosome rRNA operons PFGE |
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