| 利用GICA-PCR快速检测瓜类果斑病菌 |
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| 引用本文： | 董明明,张甜甜,魏梅生,刘爱新,朱水芳,赵文军.利用GICA-PCR快速检测瓜类果斑病菌[J].植物检疫,2011(1). |
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| 作者姓名： | 董明明 张甜甜 魏梅生 刘爱新 朱水芳 赵文军 |
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| 作者单位： | 山东农业大学;中国检验检疫科学研究院;北京化工大学; |
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| 摘 要： | 瓜类果斑病菌(Acidovorax avenae subsp.citrulli,Aac)是瓜类作物上重要的病原细菌,为我国进境植物检疫性有害生物。胶体金免疫层析试纸条方便快捷,应用广泛。该方法使用不当会出现假阳性问题,仅适用于病原菌的初筛检测。本研究将Aac胶体金免疫层析方法(GICA)与PCR技术相结合,建立了GICA-PCR检测方法。检测结果表明,该方法在蛋白与核酸2个层面上从发病西瓜叶片上检测到瓜类果斑病菌,有效解决了试纸条检测的假阳性问题,提高了瓜类果斑病菌检测的准确性,值得推广应用。
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| 关 键 词： | 瓜类细菌性果斑病菌 胶体金免疫层析 GICA-PCR 检测 |
Detection of Acidovorax avenae subsp. citrulli with GICA-PCR method |
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| Dong Mingming,Zhang Tiantian,Wei Meisheng,Liu Aixin,Zhu Shuifang,Zhao Wenjun.Detection of Acidovorax avenae subsp. citrulli with GICA-PCR method[J].Plant Quarantine,2011(1). |
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| Authors: | Dong Mingming Zhang Tiantian Wei Meisheng Liu Aixin Zhu Shuifang Zhao Wenjun |
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| Institution: | Dong Mingming1,2,Zhang Tiantian2,3,Wei Meisheng2,Liu Aixin1,Zhu Shuifang2,Zhao Wenjun2(1.Shandong Agricultural University,Tai'an 271018,China,2.Chinese Academy of Inspection and Quarantine,3.Beijing University of Chemical Technology) |
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| Abstract: | Acidovorax avenae subsp.citrulli(Aac),the cause agent of Bacterial fruit blotch in Watermelon,is a plant quarantine pest in China.Gold Immunochromatographic Assay(GICA) Strip is widely used for bacteria diagnosis because of its performing convenience and directly easy visual judging.However,the strip is only suitable for bacteria preliminary test since false positive result maybe appeared.In this research,we developed one GICA-PCR method combined the GICA and PCR assay.The test line of the GICA strip wascut... |
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| Keywords: | Acidovorax avenae subsp citrulli GICA-PCR detection |
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