瓜类细菌性果斑病菌群体感应信号分子的检测及其对致病性的影响

瓜类细菌性果斑病是国内外西瓜和甜瓜上危害最为严重的病害之一，目前除了严格的检疫措施之外，尚无十分有效的控制措施。随着细菌群体感应（Quorum sensing，QS）系统的发现和研究进展，利用“信号干扰技术”控制植物细菌病害已经成为研究热点并取得了成功应用。本文利用生物测定技术首次从瓜类细菌性果斑病菌（Acidovorax avenae subsp. citrulli）检测到群体感应信号分子（AHL），并将能够降解细菌信号分子的aiiA基因转化到瓜类细菌性果斑病菌NJF10菌株中，室内接种试验表明该转化菌株的致病性明显的减弱，证明其对果斑病菌的致病性具有调控作用。研究结果为利用信号干扰技术控制瓜类细菌性果斑病奠定了基础。

Detection of Quorum Sensing Signal Molecule of Acidovorax avenae subsp.citrulli and Its Regulation on Pathogenicity

Bacterial Fruit Blotch (BFB) of melons is one of the most serious diseases of both native and overseas water melon and muskmelon, while up to now, there is still no effective control method except strict quarantine measures. With the discovery and study progress of quorum sensing (QS), using signal interference technology to control plant bacteria diseases has been a research focus and also successfully applied. This article firstly detect signal molecules (AHL) from melons Bacterial Fruit Blotch pathogen (Acidovorax avenae subsp. citrulli) by bioassays method. Gene aiiA which can degrade AHL molecules was transformed into BFB pathogen strain NJF10. Inoculation tests showed that this transformed strain has a dramatically reduce of pathogenicity.This result lays a foundation for controlling melons Bacterial Fruit Blotch by signal interference strategy.