Enhanced pollen grain embryogenesis and plant regeneration in anther cultures of Brassica juncea cv. PR-45 |
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Authors: | Pradeep Kumar Agarwal Sant S Bhojwani |
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Institution: | (1) Department of Botany, University of Delhi, 110 007 Delhi, India |
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Abstract: | Summary Pollen grain embryogenesis in anther cultures of Brassica juncea cv. PR-45 was considerably enhanced by treating the donor plants with 4 mll-1 (v/v) of ethrel or delayed sowing of the donor plants, the latter treatment being superior. The anthers derived from plants sown about two months after the normal sowing period showed 18% androgenesis as compared to 3.5% in the control.Pollen grain embryos normally showed very poor germination (10%) on B5 or B5 containing GA3. However, ABA or cold treatment promoted normal germination of these embryos. Exposure of the embryos to 4°C for 6 days, which proved to be the best treatment, induced 66% germination of the embryos. |
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Keywords: | anther culture Brassica juncea haploids mustard |
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