西葫芦种质资源遗传多样性的RAPD分析

利用RAPD技术对国内外47份西葫芦种质材料进行遗传多样性及亲缘关系分析。从520条随机引物中筛选出30条能产生稳定多态性的引物用于RAPD分析,共扩增出367条带,其中302条表现多态性,占总数的82.29%,表明西葫芦种质资源在分子水平上具有丰富的遗传多样性,与形态学研究结果一致。聚类分析将47份西葫芦种质材料分为两大类。在第Ⅰ类群中,可将32份种质分为4个亚类;第Ⅱ类群分成5个亚类。从生长习性来看,矮生与半蔓生的亲缘关系最近;从皮色来看,白色与绿色亲缘关系最近,亲缘关系最远的为白色与花皮。

Analysis of Genetic Diversity in Summer Squash Germplasm by RAPD

In this study, RAPD analysis was applied to assess the genetic diversities and relationship with 47 internal and external summer squash（Cucurbita pepo L.）germplasm resources. Genomic DNA of 47 summer squash germplasm resources was amplified by RAPD. 30 polymorphic primers screened out of 520 random primers were used and 367 reproducible bands were produced. Among them, 302 bands（82.29 %）were polymorphic. The genetic diversity of summer squash germplasm resources was very rich. This result is consistent with morphological study. The cluster analysis divided the germplasm resources used in this study into 2 groups. The first group was divided into 4 sub-groups. The second group was divided into 5 sub-groups. The different sub-groups of summer squash had their own phenotype characteristics. For growth habit, the genetic relationship between dwarf and half-vine was the nearest. For skin color, the genetic relationship between white and green was the nearest. And the genetic relationship was the farthest between white and colored.