Aneuploids as a key for new molecular cloning strategies: development of DNA markers by microdissection using Triticum aestivum-Aegilops markgrafii chromosome addition line B |
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Authors: | Heidi Potz Veit Schubert Andreas Houben Ingo Schubert W Eberhard Weber |
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Institution: | (1) Institut für Pflanzenzüchtung und Pflanzenschutz, Martin-Luther-Universität Halle-Wittenberg, Berliner Strasse 2, D-06188 Hohenthurm, Germany;(2) Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstrasse 3, D-06466 Gatersleben, Germany |
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Abstract: | Summary We established a chromosome specific DNA library of the Aegilops markgrafii chromosome B. Eight microdissected chromosomes B obtained from a monosomic T. aestivum-Aegilops markgrafii addition line were PCR-amplified and the DNA was cloned in Escherichia coli DH5. Clones were characterized by dot blot hybridization with total Ae. markgrafii DNA. 62% of clones represented repetitive sequences and 38% low or single copy sequences. The estimated length of excised inserts varied between less than 200 bp and more than 500 bp. The average size of inserts was 310 bp.Abbreviations bp
base pairs
- DOP-PCR
degenerated oligonucleotide primed polymerase chain reaction |
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Keywords: | Aegilops markgrafii aneuploids chromosome specific library DOP-PCR microdissection molecular markers Triticum aestivum wheat |
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