Isolation and characterization of <Emphasis Type="Italic">Vibrio metschnikovii</Emphasis> causing infection in farmed <Emphasis Type="Italic">Portunus trituberculatus</Emphasis> in China |
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Authors: | Xihe Wan Hui Shen Libao Wang Yongxu Cheng |
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Institution: | (1) Laboratory of Marine Biotechnology, Jiangsu Institute of Oceanography & Marine Fisheries, 226007 Nantong, China;(2) Shanghai Ocean University, 201306 Shanghai, China |
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Abstract: | In August 2008, a massive epizootic occurred among Portunus trituberculatus reared together with Palaemon carincauda Holthuis and Sinonovacula constricta in the seawater pond of Ningang farm, Rudong district, Jiangsu province, China. The disease occurred in crabs from juveniles
to adults, and the mortality rate reached 30–40%. The diseased crabs exhibited lethargy, hepatopancreas turgidity, and elevated
levels of turbid hemolymph. A gram-negative, rod-shaped bacterium (designated as strain LGS-1) was isolated from the ascitic
fluid of the diseased and moribund crabs and was confirmed as the causative agent by our infectivity study. We conducted morphological
and biochemical characterization of LGS-1, and sequenced the 16S rRNA gene, which led us to identify the bacterium as Vibrio metschnikovii. Drug sensitivity tests showed that this pathogenic bacterium is sensitive to florfenicol, orfloxacin, and SXT, but completely
resistant to antibacterial drugs like gentamicin, erythrocin, and acheomycin. This is the first report on Vibrio metschnikovii as a virulent pathogen for Portunus trituberculatus. |
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