Screening of Optimal Differentiation Medium toLonicera edulis |
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| Authors: | Li Fu-heng Xu Qing-hua ПоляковАВ Qu Di Zhu Hui-jie Li Nan-ding Liu Zeng-bing |
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| Affiliation: | 1. College of Life Sciences, Northeast Agricultural University, Harbin 150030, China
2. All Russian Research Institute of Vegetable Crops RAAS, Ramensky District, Moscow Region, 140153, Russia |
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| Abstract: | An excellent Lonicera edulis strain, L1-8 that was bred by Northeast Institute of Geography and Agro-ecology, Chinese Academy of Sciences, was used as material in this research. The axillary buds of its dormant branches were used as explants. A four-factor and four-level orthogonal test was designed in order to choose the best differentiation medium for providing the technical support of Lonicera edulis micropropagation. The results showed that the culture medium and concentration of 6-BA were the main factors, and the best differentiation condition was MS culture medium containing 2.0 mg · L−1 6-BA, 0.3 mg · L−1 IBA and 1.5 mg · L−1 GA3. |
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| Keywords: | Lonicera edulis differentiation culture medium orthogonal test |
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