| 金属离子及低温对中华倒刺鲃精子活力的影响 |
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| 引用本文： | 张春霖,李英文,邹奇志,潘仲华.金属离子及低温对中华倒刺鲃精子活力的影响[J].西南农业大学学报,1999(3). |
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| 作者姓名： | 张春霖 李英文 邹奇志 潘仲华 |
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| 作者单位： | 西南农业大学水产系 |
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| 摘 要： | 对中华倒刺鱼巴（Ｓｐｉｎｉｂａｒｂｕｓｓｉｎｅｎｓｉｓ）精子在金属离子处理和低温保存下精子活力的影响研究表明，在ＮａＣｌ和ＫＣｌ溶液的渗透压约为３０８Ｋｐａ时，精子的活力最强；当高于６８０．１Ｋｐａ后，精子活动完全被抑制。Ｃａ，Ｍｇ浓度较高对精子有一定的损伤作用。精液稀释比为１∶１或３∶１时，在０～２℃保存的效果较好。稀释液中添加２．６４％的甘油，能延长精子的寿命，添加３％的葡萄糖对保存也有利。
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| 关 键 词： | 中华倒刺鲃 金属离子 精子—活力 |
EFFECTS OF METAL IONS AND LOW TEMPERATURE PRESERVATION ON SPERMATOZOAL MOTILITY OF SPINIBARBUS SINENSIS |
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| Zhang Chunling, Li Yingwen, Zou Qizhi, Pan Zhonghua.EFFECTS OF METAL IONS AND LOW TEMPERATURE PRESERVATION ON SPERMATOZOAL MOTILITY OF SPINIBARBUS SINENSIS[J].Journal of Southwest Agricultural University,1999(3). |
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| Authors: | Zhang Chunling Li Yingwen Zou Qizhi Pan Zhonghua |
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| Abstract: | The spermatozoal motility of spinibarbus sinensis was tested in solutions of NaCl, KCl, CaCl2, MgCl2 and in low temperature preservation. The results show: 1. in NaCl and KCl solutions the sperm obtains the highest motility at 308 Kpa of solutions' osmotic pressure, and the activity of sperm is inhibited at 680.1 Kpa. Higher concentration of CaCl2, MgCl2 in solutions does harm to the sperm. 2. the result of low temperature preservation (02) is better with a dilution of 1 to 2 and 1 to 4. 3. The sperm can live longer when 2.64% of glycerol is added in low temperature preservation. In addition, 3% addition of glucose is benificial for the preservation of sperm. |
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| Keywords: | Spinibarbus sinensis metal ions spermatozoal motility |
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