Fbxw7β, E3 ubiquitin ligase,negative regulation of primary myoblast differentiation,proliferation and migration |
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| Authors: | Kyungshin Shin Sang‐Gu Hwang Ik Joon Choi Young‐Gyu Ko Jaemin Jeong Heechung Kwon |
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| Institution: | 1. Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Science, Seoul, South Korea;2. Department of Biotechnology, Korea University, Seoul, South Korea;3. Department of Otorhinolaryngology‐Head and Neck Surgery, Korea Cancer Center Hospital, Seoul, South Korea;4. College of Life Sciences and Biotechnology, Korea University, Seoul, South Korea;5. Department of Surgery, Hanyang University College of Medicine, Seoul, South Korea |
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| Abstract: | Satellite cells attached to skeletal muscle fibers play a crucial role in skeletal muscle regeneration. During regeneration, the satellite cells proliferate, migrate to the damaged region, and fuse to each other. Although it is important to determine the cellular mechanisms controlling myoblast behavior, their regulators are not well understood. In this study, we evaluated the roles of Fbxw7 in primary myoblasts and determined its potential as a therapeutic target for muscle disease. We originally found that Fbxw7β, one of the E3 ubiquitin ligase Fbxw7 subtypes, negatively regulates differentiation, proliferation and migration of myoblasts and satellite cells on muscle fiber. However, these phenomena were not observed in myoblasts expressing a dominant‐negative, F‐box deleted Fbxw7β, mutant. Our results suggest that myoblast differentiation potential and muscle regeneration can be regulated by Fbxw7β. |
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| Keywords: | differentiation Fbxw7β migration myoblast skeletal muscle |
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