Effect of supplemented sericin on the development,cell number,cryosurvival and number of lipid droplets in cultured bovine embryos |
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| Authors: | Misa Hosoe Yasushi Inaba Yutaka Hashiyada Kei Imai Kenji Kajitani Yuichi Hasegawa Mamoru Irie Hidetoshi Teramoto Toru Takahashi Sueo Niimura |
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| Institution: | 1. National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan;2. National Livestock Breeding Center, Kato, Hokkaido, Japan;3. National Livestock Breeding Center, Nishigo, Fukushima, Japan;4. Department of Sustainable Agriculture, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan;5. National Institute of Livestock and Glassland Science, Tsukuba, Ibaraki, Japan;6. Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan;7. Faculty of Agriculture, Niigata University, Niigata, Japan |
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| Abstract: | Sericin was investigated as an alternative to fetal bovine serum (FBS) for bovine embryo culture. In vitro matured oocytes were developed using 0.05%, 0.1% or 0.15% sericin. The developmental rate, cryosurvival rate and blastulation time of these embryos were compared with those of embryos developed using 5% FBS. The number of lipid droplets was compared among the blastocysts developed using 5% FBS, using 0.05% sericin and in vivo. The rate of cleavage and blastocyst formation was similar among all groups. Blastulation occurred significantly earlier in the embryos developed using 5% FBS than in those developed using sericin at any concentration (P < 0.05). At 72 h after thawing, the cryosurvival rate of the blastocysts developed using 5% FBS and 0.05% sericin were significantly higher compared with those developed using 0.1% and 0.15% sericin (P < 0.05). The blastocysts developed using 0.05% sericin and in vivo produced a significantly fewer number of medium and large lipid droplets than those developed using 5% FBS. These results suggest that the blastocysts developed using 0.05% sericin show characteristics similar to those of the blastocysts developed in vivo and that the use of sericin as an alternative to FBS is feasible. |
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| Keywords: | cryosurvival lipid droplets sericin |
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