| 大孔树脂分离纯化葛根总黄酮工艺研究 |
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| 引用本文： | 何宇新,于杰,李玲,付超美,周亮.大孔树脂分离纯化葛根总黄酮工艺研究[J].西南农业大学学报,2006,28(6):957-960. |
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| 作者姓名： | 何宇新 于杰 李玲 付超美 周亮 |
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| 作者单位： | [1]西华大学生物工程学院,四川成都610039 [2]西南大学园艺园林学院,重庆400716 [3]成都中医药大学药学院,四川成都610075 [4]成都大学生物产业学院,四川成都610070 |
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| 基金项目： | 西华大学人才引进资助项目（R0620512） |
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| 摘 要： | 根据葛根总黄酮的理化性质，对葛根水提醇沉液采用大孔树脂进行分离纯化研究，以葛根总黄酮提取率为评价指标，筛选出大孔树脂分离纯化葛根总黄酮的最佳工艺为：选用D-100型大孔树脂，吸附速度为2BV／h，除杂洗脱用水量为4BV，洗脱剂乙醇浓度为70％，乙醇用量为3BV。
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| 关 键 词： | 葛根 葛根总黄酮 大孔树脂 |
| 文章编号： | 1000-2642（2006）06-0957-04 |
| 收稿时间： | 09 5 2006 12:00AM |
| 修稿时间： | 2006-09-05 |
STUDY ON THE TECHNOLOGY OF ISOLATION AND PURIFICATION OF GROSS ANTHOXANTHIN IN RADIX PUERARIAE WITH MACROPOROUS ADSORBING RESIN |
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| HE Yu-xin,YU Jie,LI Ling,FU Chao-mei,ZHOU Liang.STUDY ON THE TECHNOLOGY OF ISOLATION AND PURIFICATION OF GROSS ANTHOXANTHIN IN RADIX PUERARIAE WITH MACROPOROUS ADSORBING RESIN[J].Journal of Southwest Agricultural University,2006,28(6):957-960. |
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| Authors: | HE Yu-xin YU Jie LI Ling FU Chao-mei ZHOU Liang |
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| Institution: | 1. Bio -Engineering College of Xihua University, Chengdu, Sichuan 610039, China; 2. College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China; 3. Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 610075, China; 4. Bio - Engineering College of Chengdu University, Chengdu, Sichuan 610039, China |
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| Abstract: | Maeroporous adsorbing resin was used to isolate and purify the decoction of Radix puerariae. With the recovery rate of gross anthoxanthin as the criterion of evaluation, the optimum conditions for isolation and purification were identified, i.e. D - 100 type macroporous adsorbing resin, adsorbing rate 2BV/h, water volume for removing impurities 4 BV, concentration of the eluent ethanol 70%, and volume of ethanol 3 BV. |
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| Keywords: | Radix puerariae gross anthoxanthin in Radix puerariae macroporous adsorbing resin |
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