Development of an enzyme-linked immunosorbent assay to detect serum antibody to chicken anemia agent |
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| Authors: | D Todd D P Mackie K A Mawhinney T J Connor F McNeilly M S McNulty |
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| Institution: | Veterinary Research Laboratories, Stormont, Belfast, Northern Ireland. |
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| Abstract: | An enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to chicken anemia agent (CAA) has been developed. This test utilizes a CAA-specific mouse monoclonal antibody to selectively capture virus antigen. Chicken antibodies to CAA bind to the captured antigen and are detected with horseradish peroxidase-labeled anti-chicken immunoglobulin using a conventional indirect ELISA protocol. When 388 chicken sera from specific-pathogen-free and commercial flocks from the United Kingdom, West Germany, the United States and Australia were examined, 98.5% agreement was obtained between the results of the ELISA and the indirect immunofluorescence assay. This ELISA should have worldwide application in testing SPF and commercial chicken flocks for CAA antibodies. |
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