Binding patterns of five monoclonal antibodies to Actinobacillus (Haemophilus) pleuropneumoniae |
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| Authors: | J Lida I M Smith |
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| Institution: | Department of Microbiology and Parasitology, Royal Veterinary College, University of London. |
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| Abstract: | Five monoclonal antibodies were obtained after immunising mice with superficial antigens of three strains representing serovars 1 to 3 of Actinobacillus (Haemophilus) pleuro-pneumoniae. When tested in ELISA against the standard strains representing serovars 1 to 10, the monoclonal antibody raised against the standard strain of serovar 1 reacted only with that strain. Of the three monoclonal antibodies raised against the standard strain of serovar 3, one reacted with serovars 3 and 8 only, another with serovar 7 only and the third with the strains representing serovars 7, 9 and 10. The monoclonal antibodies produced with the serovar 2 strain also reacted with a wide spectrum of strains, representing serovars 7 to 10. |
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