| 发根农杆菌侵染大豆产生发根的研究 |
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| 引用本文： | 李泉木,朱筠,曹越平,.发根农杆菌侵染大豆产生发根的研究[J].上海交通大学学报(农业科学版),2012,30(1):55-60. |
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| 作者姓名： | 李泉木 朱筠 曹越平 |
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| 作者单位： | 1. 上海交通大学农业与生物学院,上海200240;辽宁省农业科学院所,沈阳110161
2. 上海交通大学农业与生物学院,上海,200240 |
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| 基金项目： | 国家转基因重大专项(2008ZX8004-001) |
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| 摘 要： | 利用A4发根农杆菌,对不同大豆品种的无菌苗采用针刺的方法对子叶节部位进行接种,诱导毛状根产生,测定毛状根中大豆苷元的含量。结果表明,以合丰35为受体材料,毛状根诱导率最高,为58%,明显高于其他处理。发根中大豆苷元的含量是正常无菌苗和大田幼苗根中含量的2倍。
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| 关 键 词： | 大豆 发根农杆菌 发根 大豆苷元 |
Hairy Root Induced by Agrobacterium rhizogenes in Soybean |
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| LI Quan-mu
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ZHU Jun
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CAO Yue-ping.Hairy Root Induced by Agrobacterium rhizogenes in Soybean[J].Journal of Shanghai Jiaotong University (Agricultural Science),2012,30(1):55-60. |
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| Authors: | LI Quan-mu ZHU Jun CAO Yue-ping |
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| Institution: | 1(1.School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China; 2.Liaoning Academy of Agricultral Sciences,Shenyang 110161,Chnia) |
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| Abstract: | 12 soybean materials were infected by Agrobacterium rhizogenes A4,and daidzein content was determined in hairy roots which were induced by inoculating Agrobacterium rhizogenes A4."Hefeng 35" was tested to be the best receptor among the 12 materials.Inoculating by needle on soybean cotyledon node was the best method for inducing hairy roots,the induced rate was 58%.Daidzein content in hairy roots was 2 times of that in seedling cultivated in medium and field. |
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| Keywords: | soybean Agrobacterium rhizogenes hairy root daidzein |
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