濒危植物沼泽小叶桦组织培养技术及其在上海地区的中试

为快速获得大量沼泽小叶桦苗,通过对茎尖进行丛生芽诱导、生根、移栽,成功获得了沼泽小叶桦组培苗。研究了沼泽小叶桦外植体不同灭菌方法、培养基等因素对沼泽小叶桦组培的影响。结果表明,10%次氯酸钠作为外植体消毒剂优于0.1%升汞,适宜的丛生芽诱导增殖培养基为MS+0.6mg/L 6-BA+3%蔗糖+0.7%琼脂,最佳生根培养基为MS+3%蔗糖+0.7%琼脂。无菌苗移栽在蛭石:珍珠岩:草炭=3:3:4的介质中,成活率达100%,生长良好。

Tissue Culture of Endangered Plant Betula microphylla var.paludosa and Its Pilotscale Experiment in Shanghai Area

A large number of seedlings of Betula microphylla var.paludosa was achieved by inducing adventitious buds,rooting and transferring.The effects of different disinfection methods,culture medium and proportions of transferring media on explants Betula microphylla var.paludosa were investigated.The result showed that the disinfection effect of 10% sodium hypochlorite was better than 0.1% mercuric chloride.The appropriate culture medium for adventitious bud inducing proliferation was MS + 0.6 mg/L 6-BA + 30 g/L sucrose + 7 g/L agar.The optimized rooting medium was MS + 30 g/L sucrose + 7 g/L agar.The most effective medium for livability of asepsis sprout transferring of Betula microphylla var.paludosa was vermiculite:perlite:peat = 3:3:4,and its surviving rate reached 100%.Tissue culture seedlings grew well in Shanghai area.