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| 茶树花发育相关的一个钙依赖蛋白激酶基因的克隆与表达分析 | |
| 引用本文： | 陈暄,汤茶琴,邹中伟,杨亦扬,房婉萍,王玉花,黎星辉.茶树花发育相关的一个钙依赖蛋白激酶基因的克隆与表达分析[J].茶叶科学,2009,29(1). |
| 作者姓名： | 陈暄 汤茶琴 邹中伟 杨亦扬 房婉萍 王玉花 黎星辉 |
| 作者单位： | 1. 南京农业大学茶叶科学研究所,江苏,南京,210095 2. 南京农业大学茶叶科学研究所,江苏,南京,210095;无锡市茶叶品种研究所,江苏,无锡,214063 3. 无锡市茶叶品种研究所,江苏,无锡,214063 |
| 基金项目： | 教育部高等学校博士学科点专项科研基金，南京农业大学青年科技创新基金，江苏省农业三项工程项目，江苏省科技攻关项目，国家自然科学基金 |
| 摘 要： | 应用cDNA-AFLP(Amplified Fragment Length Polymorphism)技术分析了结实率差异显著的龙井43和大叶乌龙两个茶树品种在花蕾发育过程中基因表达的差异。从获得的差异图谱中,克隆得到一个与茶树花发育相关的钙依赖蛋白激酶基因片段,然后用RCAE方法扩增获得其cDNA全长序列,命名为茶树TCK(Camellia sinensis calcium-dependent protein kinase)基因,GenBank登录号EU732607。该基因cDNA序列全长2281bp,编码760个氨基酸。用RT-PCR方法进一步研究该基因的功能,检测其表达特异性,结果表明该基因只在茶树花蕾发育后期特异表达,在叶、花蕾发育早期均无表达,提示TCK基因可能在茶树花发育过程中发挥重要作用。 |
| 关 键 词： | 茶树 花发育 钙依赖蛋白激酶基因 克隆 特异表达 |
Molecular Cloning and Expression of a Flowering-related Calcium-dependent Protein Kinase Gene in Tea Plant [Camellia sinensis (L.)O.Kuntze] |
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| CHEN Xuan,TANG Cha-qin,ZOU Zhong-wei,YANG Yi-yang,FANG Wan-ping,WANG Yu-hua,LI Xing-hui.Molecular Cloning and Expression of a Flowering-related Calcium-dependent Protein Kinase Gene in Tea Plant [Camellia sinensis (L.)O.Kuntze][J].Journal of Tea Science,2009,29(1). | |
| Authors: | CHEN Xuan TANG Cha-qin ZOU Zhong-wei YANG Yi-yang FANG Wan-ping WANG Yu-hua LI Xing-hui |
| Institution: | 1. Tea Research Institute;Nanjing Agricultural University;Nanjing 210095;China;2. Tea variety Research Institute of Wuxi;Wuxi;214063;China |
| Abstract: | To have an insight into the comprehensive molecular characteristics relating to the flower development mechanisms of tea plant Camellia sinensis (L.)O.Kuntze], cDNA-AFLP (Amplified Fragment Length Polymorphism) was performed within two tea plant cultivars, Longjin43 and Daye Wulong. A cDNA fragment encoding a calcium-dependent protein kinase gene was isolated and identified. The complete cDNA sequence was cloned by RACE (rapid amplification of cDNA ends), named TCK(Camellia sinensis calcium-dependent protei... |
| Keywords: | Camellia sinensis flower development calcium-dependent protein kinase gene cloning differential expression |
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