| Characterization and Comparison of Ochratoxin A-Ovalbumin (OTA-OVA) Conjugation by Three Methods |
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| 作者姓名： | WANG Xi-chun ;BAO Ming ;WU Jin-jie ;LUO Ying ;MA Liang-you ;WANG Wing ;ZHANG Ai-hua ;HE Cheng-hua ;ZHANG Hai-bin |
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| 作者单位： | [1]College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, BR. China; [2]Aquatic Technology Promotion Station of Anhui Province, Hefei 230066, P.R. China; [3]College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R. China |
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| 基金项目： | Acknowledgements This work was supported by the National High-Technology Research and Development Program of China （2007AA10Z429） and the Stabling and Introducing Talents Fund Program of Anhui Agricultural University, China （WD2011-17）. |
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| 摘 要： | In order to generate an antibody against a small hapten molecule, the hapten is cross-linked with carrier protein to make it immunogenic. In this study, the hapten （ochratoxin A, OTA） was coupled to ovalbumin （OVA） by an active ester reaction. To develop a technique for detecting the conjugation, the hapten-protein conjugate （OTA-OVA） was characterized thoroughly by immunoarray technology, ultraviolet （UV） spectroscopy and matrix-assisted laser desorption ionization time-of-flight mass spectrometry （MALDI-TOF-MS）, respectively. The molecular weight of OTA-OVA was 50 350.141 Da, and the molecular weight of OVA was 44 887.506 Da, which were determined by MALDI-TOF-MS, respectively. In OTA-OVA, the molecular coupling ratio was 13：1 by MALDI-TOF-MS while the molecular coupling ratio was 10：1 by UV. In this experiment, UV and MALDI-TOF-MS were selected as the efficient methods to evaluate the cross-linking effect and calculate the molecular coupling ratio.
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| 关 键 词： | 赭曲霉毒素A MALDI-TOF-MS 卵清蛋白 基质辅助激光解吸电离质谱 共轭 抗原分子 表征 半抗原 |
Characterization and Comparison of Ochratoxin A-Ovalbumin （OTA-OVA） Conjugation by Three Methods |
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| WANG Xi-chun,;BAO Ming,;WU Jin-jie,;LUO Ying,;MA Liang-you,;WANG Wing,;ZHANG Ai-hua,;HE Cheng-hua,;ZHANG Hai-bin.Characterization and Comparison of Ochratoxin A-Ovalbumin (OTA-OVA) Conjugation by Three Methods[J].Agricultural Sciences in China,2014(5):1130-1136. |
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| Abstract: | ochratoxin A, conjugated antigen, cartier protein, coupling ratio |
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| Keywords: | ochratoxin A conjugated antigen cartier protein coupling ratio |
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