猪和绵羊组织器官乳酸脱氢酶同工酶酶谱比较

应用聚丙烯酰胺凝胶电泳法对猪和绵羊的心、肝、脾、肺、肾、胰、小肠７种组织器官及血清的乳酸脱氢酶同工酶酶谱进行比较研究，结果发现：①猪和绵羊各组织器官中ＬＤＨ同工酶共有５种区带，猪的ＬＤＨ３同工酶还可分为两条亚带；②猪和绵羊的肺、肾、小肠、胰、血清中ＬＤＨ区带数相同，其它组织器官各不相同；③猪和绵羊ＬＤＨ１同工酶的泳动速度最快，迁移率分别为５３．６４％、４１．８２％；ＬＤＨ５同工酶的泳动速度最慢，迁

Comparative study on LDH Isozyme-spectra of Tissues and or gans In Pigs and Sheep

The LDH isozyme-spectra of 7 kinds of tissues and organs including heart,liver,spleen,lung,kidney,pancreas,small intestine and serum between pigs and sheep were compara-tively studied by means of a polyacrylamide gel electrophoresis.The results showed as follows:(1)The LDH isozyme-spectra of tissues and organs in both pigs and sheep composed of 5 bands,and the LDH 3 isozyme in pigs consisted of 2 sub-bands;(2)There was same number of bands oflung,kidney,small intestine,pancreas and serum in both pigs and sheep;(3)The mobile speeds ofLDH1 isozyme in both pigs and sheep were the fastest and their mobility rate were 53.64%and41.82%respectively,those of LDH5 isozyme were the slowest,and their mobility rate were10.0%and 16. 36%. respectively.