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DNA sequence analysis of ribosomal ITS region 1 of Phytophthora vignae f. sp. adzukicola, the pathogen that causes stem rot of adzuki bean
Authors:Norio Kondo  Ayumi Notsu  Shohei Fujita  Hisanori Shimada  Shigeo Naito
Institution:(1) Laboratory of Plant Pathology, Graduate School of Agriculture, Hokkaido University, Kita 9 Nishi 9, Sapporo 060-8589, Japan;(2) Tokachi Agricultural Experiment Station, Memuro, Japan
Abstract:Sequences of the internal transcribed spacer (ITS) region 1 were used to examine the phylogenetic relationships among races of 19 isolates of Phytophthora vignae f. sp. adzukicola and between this forma specialis and three isolates of the closely related P. vignae f. sp. vignae. The ITS 1 sequences were highly conserved (> 98.7% similarity) among representatives of both formae speciales groups. The results of this study indicate that P. vignae is a monophyletic group. The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession nos. AB120062–AB120080 and AB120122
Keywords:Phytophthora vignae f  sp  adzukicola  Phytophthora vignae f  sp  vignae  ITS sequence  Adzuki bean  Cowpea
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