2006～2008年华东地区新城疫病毒HN基因序列分析

为研究新城疫病毒（Newcastle disease virus，NDV）的HN（血凝素-神经氨酸酶，hemagglutinin—neuraminidase）基因分子流行病学规律，利用RT—PCR扩增了2006～2008年间分离自我国华东地区的43株NDV的HN基因片段。基因序列分析显示，分离株中基因Ⅰ型2株，基因Ⅱ型3株，基因Ⅵ型1株，其它37株都属于基因Ⅶd亚型。根据遗传发生进化树可将基因Ⅶd亚型进一步分为Ⅶd1和Ⅶd2两个亚型，基因Ⅶd1亚型HN蛋白的第102、118、443位氨基酸分别为T，A和T，而基因Ⅶd2亚型则分别为I，E和M。另外，研究表明HN蛋白线性表位发生E347K突变的变异株的分离率在我国呈上升趋势。

SEQUENCE ANALYSIS OF HEMAGGLUTININ-NEURAMINIDASE GENES OF NEWCASTLE DISEASE VIRUS ISOLATES IN EASTERN CHINA DURING 2006-2008

In order to investigate the molecular epidemiology of Newcastle disease virus in China, forty-three Newcastle disease viruses （NDV） isolated from Eastern China during 2006 -2008 were characterized phylogeniclly. Genetic analysis based on the HN gene sequences revealed that the isolates were classified into four genotypes： 2 isolates belonged to genotype Ⅰ, 3 to genotype Ⅱ , 1 to genotype Ⅵ and 37 to genotype Ⅶd. The genotype Ⅶd NDVs could be further classified into two subgroup, Ⅶd 1 and Ⅶd 2. Compared of Ⅶd 1 viruses, the viruses in Ⅶd 2 presented the three amino acid sequence mutations of T102I, A118E and T443M in HN protein. Besides, the results showed that the separation rate of the E347K mutation isolation in the HN protein linear epitope in China is increasing with time.