麦穗鱼鳍条组织培养及染色体Ag—NORs和C-带研究

采用组织块贴壁培养法，探索了麦穗鱼（Pseudorasbora parva）鳍组织细胞短期培养方法，同时对其染色体Ag-NORs和C-带进行了初步研究。结果显示：1）麦穗鱼鳍组织培养的最适培养基为MEM培养基，细胞在组织块贴壁后第6天迁出，经传代后细胞形态呈成纤维状；2）其染色体具有1对Ag-NORs，位于第23对染色体短臂末端，其NORs具多态性，未见Ag-NORs联合现象；3）其染色体C-带主要为端粒和着丝点C-带，少数为居间C-带，其中第23对染色体的短臂大部分呈现C-带强阳性，与Ag-NORs区域对应。

Studies on fin tissues cultivation, Ag-NORs and C-banding patterns of Pseudorasbora parva

We investigated short-term fin tissues cultivation of Pseudorasbora parva by explant technique, and studied its Ag-NORs, and C-banding patterns at the same time. Results are as follows： 1） Cells from the fin tissues of P.parva migrate out around tissues at 6thday through MEM medium, the most suitable one, and are fibroblast-like during cultivation; 2） A pair of Ag-NORs is found on the end of no.23 chromosome short arm, and the NORs are polymorphic, while no Ag-NORs combination has happened in this fish. 3） C-bands are located mainly in the terminal and centromere regions of chromosomes, and a few in the interstitial regions. In particular, most of the short arm on chromosome No.23 shows strong positive C-bands, which is corresponded with Ag-NORs region.