| DNA shuffling技术改造Bt基因的水稻转化研究 |
| |
| 引用本文： | 王娟,张小琼,林拥军.DNA shuffling技术改造Bt基因的水稻转化研究[J].分子植物育种,2008,6(5). |
| |
| 作者姓名： | 王娟 张小琼 林拥军 |
| |
| 作者单位： | 华中农业大学作物遗传改良国家重点实验室,武汉,430070 |
| |
| 基金项目： | 863计划重点研究项目(2007AA10Z188)资助 |
| |
| 摘 要： | 本试验挑选了以cry1Ab、cry1Ac、cry9C、cry1C和cry2A等5个基因经DNA family-shuffling改造后的不同组的14个杀虫活性有显著提高的重组胁基因，通过农杆菌介导法转入水稻品种中花11中。各基因均获得80棵以上独立转化植株，通过对转化植株的PCR阳性检测，获得了约75％的阳性单株。Southern结果显示转化植株的拷贝数分别是1～12不等，单拷贝率为12％；Northern分析其表达量，结果显示除了很少发生基因沉默外其余单株均表达了胁基因，但是植株间表达量差异较大：随机挑选阳性植株喂食一龄的二化螟进行生测实验，与原始基因相比，14个重组反基因均表现出了较高的毒性。
|
| 关 键 词： | Bt基因 水稻 转基因植株 分子检测 |
The Study on Rice Transformation of Recombined Bt Genes Derived from DNA Shuffling Library |
| |
| Wang Juan,Zhang Xiaoqiong,Lin Yongjun.The Study on Rice Transformation of Recombined Bt Genes Derived from DNA Shuffling Library[J].Molecular Plant Breeding,2008,6(5). |
| |
| Authors: | Wang Juan Zhang Xiaoqiong Lin Yongjun |
| |
| Abstract: | In this study,14 mutant genes with higher toxicity were selected from a recombined cry library which was constructed by DNA family-shuffling of cry1Ab,cry1Ac,cry9C,cry1C and cry2A,and were introduced into Zhonghua 11.More than 80 independent plants were got for every gene,PCR analysis showed that about 75% of these plants were transformants.These transformants were detected by Southern blotting,results showed that they had the copy number ranging from 1 to 12,and 12% of them were single-copy insertion;RNA e... |
| |
| Keywords: | Bt gene Oryza sativa L Transgenic plant Molecular identification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
