米仔兰中环戊烷并苯呋喃化学成分及细胞毒活性研究

目的]对米仔兰中环戊烷并苯呋喃化学成分及细胞毒活性进行研究。方法]采用硅胶、Sephadex LH-20柱层析及制备薄层色谱等分离方法,对米仔兰(Aglaia odorata Lour.)的化学成分进行研究,通过1H NMR、13C NMR和MS等波谱技术鉴定化合物的结构,并采用MMT法对其细胞毒活性进行测试。结果]从米仔兰枝叶中分离得到6个包含环戊烷并苯呋喃结构的化合物,鉴定分别是roca-glamide、aglaroxin E、3'-Hydroxyrocaglafolin、3'-Hydroxyaglamide、rocaglaol和cyclorocaglamide。在极低的浓度下,化合物1～5分别对肿瘤细胞株A-539和MCF-7显示出细胞毒活性。结论]化合物2和6在米仔兰植物中首次被发现;化合物1～5具有较好的细胞毒活性。

Cyclopenta[b] benzofurans Chemical Constituents and Their Cytotoxicity Activity from Aglaia odorata Lour.

Objective] To study cyclopentab]benzofurans chemical constituents and Their cytotoxicity activity from Aglaia odorata Lour..Method] Column chromatgraphies of silica gel,Sephadex LH-2 and preparative TLC were used to isolate and purify the chemical constituents from Aglaia odorata Lour.The structures were identified by 1H NMR,13C NMR,MS techniques,MMT method was adopted to test cytotoxicity activity.Result] Six compounds including cyclopentab]benzofuran from the tiwgs and leaves of Aglaia odorata Lour.were separated,including rocaglamide,aglaroxin E,3'-Hydroxyrocaglafolin,3'-Hydroxyaglamide,rocaglaol and cyclorocaglamide.The result indicated that compounds 1-5 exhibit significant cytotoxicity against A-549 and MCF-7.Conclusion] Compound 2 and 6 were found firstly in Aglaia odorata Lour.;compound 1-5 has good cytotoxicity activity.