水稻不育系培矮64S的空间诱变效应及后代的SSR分析

对经过卫星搭载的培矮64S种子及后代进行了有关性状研究和SSR标记分析,结果表明:培矮64S空间诱变后代SP1代发芽率、存苗率、株高、抽穗期、株叶型的性状与对照相比没有明显变化;但SP2代在株高、粒型和育性等性状上发生了变异;从SP3代中获得柱头比培矮64S明显增大的变异株系,这对于改良培矮64S的异交结实率和提高制种产量具有重要意义。此外,对10个SP3变异株进行SSR分析结果表明,扩增片段数目变化约占55.21%,扩增片段分子量变化约占44.79%,空间诱变引起的变异可能是以DNA缺失-重复为主。SSR座位的变异频率平均为23.33%,变异座位在水稻基因组中是随机分布的。

EFFECTS OF SPACE MUTATION ON P(T)GMS-LINE PEI′AI 64S OF RICE AND SSR ANALYSIS OF MUTANTS

Some traits of the progenies SP_1,SP_2 and SP_3derived from Pei′ai 64S treated by space flight of recoverable satellite were studied,and polymorphism analysis of SSR for the mutants were carried out.The results showed that no difference between the treated Pei'ai64S and the original one in germination rate,survival seedling rate,plant height,heading date and plant type in SP_1was observed,but there was significant difference in fertility,plant height and grain shape in generation of SP_2.Some mutants with much bigger stigma than that of the original Pei′ai 64S were obtained in SP_3,and it would be useful in improving the out-crossing rate and enhancing the seed production of Pei′ai 64S.In addition,SSR marker analysis for ten mutants indicated that the average mutant frequency of SSR loci for the mutants was 23.33% and the mutant loci randomly distributed along the chromosomes.Among the mutant SSR loci about 55.21% showed changes in band number and 44.78% in molecular weight.DNA deficiency and duplication might be the main reason of mutation caused by space flight.