| 传染性法氏囊病病毒VP2基因高变区序列分析 |
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| 引用本文： | 王永山,周宗安,高健. 传染性法氏囊病病毒VP2基因高变区序列分析[J]. 中国预防兽医学报, 2000, 22(2): 136-139 |
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| 作者姓名： | 王永山 周宗安 高健 |
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| 作者单位： | [1]南京军区军事医学研究所 [2]上海市肿瘤研究所癌基因与相关基因国家重点实验室 |
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| 基金项目： | 江苏省自然科学基金!(BK971 87) |
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| 摘 要： | 根据传染性法氏囊病病毒（ＩＢＤＶ）ＶＰ２基因ＣＤＮＡ序列，在ＶＰ２基因高变区设计一对引物，用ＲＴ－ＰＣＲ方法扩增ＩＢＤＶ分离株ＪＳ３和ＪＳ４。将扩增片段克隆后以双脱氧链末端终止法测定核苷酸旬。ＪＳ３和ＪＳ４的同源性最高达９８％。与已发表的ｖｖＩＢＤＶ，ＩＢＤＶ变异要ＢＤＶ经典株为ＩＢＤＶ弱毒株核苷酸序列的同尖拨天９２￣９８％之间，根据ＩＢＤＶ的大ＯＲＦ推导出该片段蛋白的氨基酸序更，ＪＳ３和ＪＳ４的
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| 关 键 词： | 传染性法氏囊病 病毒 VP2基因高变区 同源性分析 |
| 修稿时间： | 1999－09－06 |
Analysis of the Sequences of Variable Region in VP2 Gene of Infectious Bursal Disease Viruses |
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| WANG Yongshan ,ZHOU Zongan ,GAO Jian ,DIAO Zhenyu ,DENG Xiaozhao ,ZHAO Xintai ,LI Jinjun ,LUO Hanlu. Analysis of the Sequences of Variable Region in VP2 Gene of Infectious Bursal Disease Viruses[J]. Chinese Journal of Preventive Veterinary Medicine, 2000, 22(2): 136-139 |
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| Authors: | WANG Yongshan ZHOU Zongan GAO Jian DIAO Zhenyu DENG Xiaozhao ZHAO Xintai LI Jinjun LUO Hanlu |
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| Affiliation: | WANG Yongshan 1,ZHOU Zongan 1,GAO Jian 1,DIAO Zhenyu 1,DENG Xiaozhao 1,ZHAO Xintai 2,LI Jinjun 2,LUO Hanlu 3 |
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| Abstract: | According to the published cDNA sequences of infectious bursal disease viruses(IBDV),a pair of primers to variable region of VP2 gene were designed for cDNA synthesis and PCR amplification of two different isolates JS3 and JS4 of IBDV.The amplified fragments by RT_PCR technique were cloned,and sequenced by the sanger dideoxy_mediated chain_termination method.The homology of the sequences of JS3 and JS4 was 98%.The identity of JS3 and JS4 with vvIBDV,variant IBDV,classical IBDV and attenuated IBDV published in GenBank databases ranged from 92% to 98%. The homology of the deduced amino acid sequences based on the ORF of JS3 and JS4 was 97%.The identity of JS3 and JS4 with above strains varied from 91% to 97%.The heptapeptide of IBDV was highly conserved in virulent strains,but mutated in attenuated strains. |
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| Keywords: | Infectious bursal disease virus Variable region of VP2 gene Analysis of homology |
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