Identification of Bt-transgenic Rice Plants for Resistance to Stripe Stem Borer (Chilo suppressalis) and Genetic Analysis of the Transgenes |
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Authors: | ZHU Chang-Xiang YAO Fang-yin LI Guang-xian WEN Fu-jiang |
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Affiliation: | 1. College of Life Sciences, Shandong Agricultural University, Taian 271018, P.R. China 2. Shandong Rice Research Institute, Jining 272077, P. R . China |
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Abstract: | Resistance to herbicide Basta was used to identify Bt-transgenic rice plants and the progenies of crosses between the Bt-transgenic rice and the rice varieties cultivated in the Huang Huai area of China. The results demonstrated that the Basta-positive rice plants were highly resistant to stripe stem borer (Chilo suppressalis ) both in the laboratory and field tests. Both cryIA (b) and bar genes were expressed and co-inherited in both selfing and crossing progenies. Mendelian segregation of the marker gene bar was observed in F2 and BC1 progenies. The results implicates that it is possible to transfer cryIA (b) gene into other cultivated varieties through crosses and back crosses. |
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Keywords: | Bt-transgenic plants Basta-resistance Resistance to stripe stem borer Genetic analysis Chilo suppressalis |
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