| 超氧化物歧化酶脂质体的制备及性质研究 |
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| 引用本文： | 何苡,吕全建,童岩.超氧化物歧化酶脂质体的制备及性质研究[J].郑州牧业工程高等专科学校学报,1999(4). |
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| 作者姓名： | 何苡 吕全建 童岩 |
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| 作者单位： | 河南省人民医院,郑州牧专基础部,郑州牧专基础部 郑州 450003,郑州 450008,郑州 450008 |
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| 摘 要： | 本文采用薄膜法制备LA-SOD脂质体,其中磷脂酰胆碱:胆固醇:心磷脂:LA-SOD配比为6∶2∶2∶1,制剂为冻干型,水化后振荡即可重新形成脂质体。其活性包封率为95％以上,粒径约为0.66μm。产品稳定性高,在4℃避光贮藏8个月,其活性无明显改变。
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| 关 键 词： | 修饰超氧化物歧化酶 脂质体 包封 |
Study on preparation of liposome -entrapped repaired -superoxide dismutase |
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| He Yi,Lu Quanjian Tong Yan.Study on preparation of liposome -entrapped repaired -superoxide dismutase[J].Journal of Zhengzhou College of Animal Husbandry Engineering,1999(4). |
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| Authors: | He Yi Lu Quanjian Tong Yan |
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| Institution: | He Yi~1,Lu Quanjian~2 Tong Yan~2 |
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| Abstract: | In this study, preparation of liposome-entrapped laurate repaired superoxide dismutase (LA-SOD)was carried out according to the"film"technique. Liposome containing phosphatidylcholine, cholesterol, cardiolipid and LA-(SOD)(in a 6: 2: 2: 1 mole ratio, repectively) was prepared. LA-SOD liposome was freeze-dried upon reconsititution with distilled water. The extent encapsule of the LA-SOD liposome was more than 965%. Stability test showed the LA-SOD liposome has higher stable. The retained activity of SOD did not changed after 8 months stored at 4 C in dark. |
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| Keywords: | liposomej encapsule repaired -superoxide dismutase |
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