| 蛹虫草小麦培养基中虫草素提取工艺研究 |
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| 引用本文： | 谭琪明,何珺,文庭池,康冀川.蛹虫草小麦培养基中虫草素提取工艺研究[J].贵州大学学报(农业与生物科学版),2011,30(4):357-361. |
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| 作者姓名： | 谭琪明 何珺 文庭池 康冀川 |
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| 作者单位： | 1. 贵州大学生命科学学院,贵州贵阳,550025
2. 贵州大学贵州省生化工程中心,贵州贵阳,550025 |
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| 基金项目： | 贵州省“十二五”农业科技攻关项目[黔科合NY字（2011）3054号];贵州省教育厅自然科学研究项目[黔教科（2009）0129号] |
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| 摘 要： | 通过对比提取溶剂、料液比、温度、pH值及时间对提取蛹虫草小麦培养基中虫草素的影响,以确定虫草素提取最佳工艺参数.结果表明:最佳提取参数为水提取、pH值5,料液比1:50、温度70℃、时间3h.该方法从蛹虫草小麦培养基中提取虫草素,提取率可达94.87％.
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| 关 键 词： | 蛹虫草小麦培养基 虫草素 提取 |
Extraction Protocol of Cordycepin from Cordyceps Wheat Medium |
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| TAN Qi-ming,HE Jun,WEN Tin-chi,KANG Ji-chuan.Extraction Protocol of Cordycepin from Cordyceps Wheat Medium[J].Journal of Mountain Agriculture & Biology,2011,30(4):357-361. |
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| Authors: | TAN Qi-ming HE Jun WEN Tin-chi KANG Ji-chuan |
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| Institution: | 1. Guizhou Province Biochemistry Engineering Center, Guizhou Univerity , Guiyang Gtdzhou 550025, China; 2. College of Life Sciences, Guiyang Guizhou 550003, China) |
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| Abstract: | To maximize the extraction efficiency of cordycepin from Cordyceps, the extraction procedure was optimized via modifying wheat medium including different extraction solution, rate of mater/al to solvent, temperature, pH and time. The result revealed that the optimum extraetion parameters were as follows： water as extraetion solution, pH 5, ratio of material to liquid 1： 50, 70℃, and 3 h, by which cordycepin might be extracted from Cordyceps conveniently and efficiently, and the extraction rate was as high as 94.87%. |
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| Keywords: | Cordyceps wheat medium cordycepin extraction |
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