Characterization of anther culture-derived cell suspensions exclusively regenerating green plantlets in wheat (Triticum aestivum L.) |
| |
Authors: | Ebiamadon Andi Brisibe Annette Olesen Sven Bode Andersen |
| |
Institution: | (1) Section of Plant Breeding and Biotechnology, Department of Agricultural Sciences, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, Copenhagen, DK - 1871, Denmark |
| |
Abstract: | A reproducible procedure for deriving highly regenerable cell suspensions that can readily and consistently regenerate green
plantlets in wheat is described. Initiation and selection of the right type of callus from anther cultures, which consisted
of friable early embryogenic portions that can easily disperse in liquid medium was important for the establishment of rapidly
growing embryogenic suspensions. Using this type of inoculum no significant variation between three different independent
replications was noted when cell suspensions from eleven specially recombined doubled haploid lines were maintained on General
medium supplemented with dicamba and a predominance of amino acid nitrogen. This approach also enhanced a long-term embryogenic
competence of the cell cultures, with some of the suspensions retaining their morphogenic capacity over a period of more than
15 months. Depending on the medium composition high frequencies of embryogenesis (over 70%) and green plantlet regeneration
(repeatedly producing 90–100% of green regenerants) were obtained from the cell aggregates for most of the embryogenic cell
lines. Potential advantages of anther culture-derived embryogenic cell suspensions for transformation purposes are the high
number of cell lines which can be established routinely and the apparent maintenance of a stable haploid genome by the regenerants
in culture. It is anticipated that an increased use of anther or microspore derived doubled haploid techniques in future wheat
breeding programmes may favour selection in the breeding material of plant types generally responsive to such protocols.
This revised version was published online in July 2006 with corrections to the Cover Date. |
| |
Keywords: | Triticum aestivum albinism breeding materials cell suspensions dicamba doubled haploid lines |
本文献已被 SpringerLink 等数据库收录! |