Plackett-Burman设计法筛选影响灵芝富锌能力的主要培养基组分 |
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引用本文: | 毕静莹,卢丽娟,秦义,樊明涛. Plackett-Burman设计法筛选影响灵芝富锌能力的主要培养基组分[J]. 安徽农业科学, 2010, 38(10): 5335-5337 |
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作者姓名: | 毕静莹 卢丽娟 秦义 樊明涛 |
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作者单位: | 1. 宁夏职业技术学院生物工程系,宁夏,银川,750002;西北农林科技大学食品科学与工程学院,陕西,杨凌,712100 2. 郑州轻工业学院,河南郑州,450002 3. 西北农林科技大学食品科学与工程学院,陕西,杨凌,712100 |
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摘 要: | [目的]筛选出影响灵芝富锌率的关键营养因素,从而为进一步的优化分析提供实践基础与理论指导。[方法]在对16种营养物质进行单因素试验的基础上,进一步利用Plackett-Burman设计法筛选出影响灵芝富锌率的关键营养因素。[结果]从16种营养物质中筛选出玉米粉、豆粕和KH2PO4是影响灵芝富锌能力的主要因素,其较优水平为玉米粉35.0g/L、豆粕7.0g/L和KH2PO41.0g/L。在以上述3种营养物质作为培养基组分的条件下,灵芝富锌率最高可达25.1%。[结论]利用Plackett-Burman设计法筛选出的关键营养因素,提高了灵芝富锌率,证明了该方法经济有效。
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关 键 词: | 灵芝 富锌率 培养基 Plackett-Burman设计 |
Screening of Main Component in the Medium Impacting the Ability of the Zinc-richening Ganoderma lucidum with the Method of Plackett-Burman Design |
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Affiliation: | BI Jing-ying et al (Department of Biological Engineering,Ningxia Vocational and Technical College,Yinchuan,Ningxia 750002 ) |
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Abstract: | [Objective] The basis of practice and the theoretical guidance to the further optimal analysis of key nutritional factors impacting the rate of zinc-richening in Ganoderma lucidum was provided through the screening experiment. [Method] The key nutritional factors impacting the rate of zinc-richening in Ganoderma lucidum were further screened out with the method of Plackett-Burman Design based on the single factor-testing of 16 kinds of nutritious substances. [Results] The main factors such as corn flour, soybean meal, and KH_2PO_4 affecting the ability of zinc-richened Ganoderma lucidum were screened out from 16 nutrients and the better level of corn flour was 35.0 g/L; soybean meal, 7.0 g/L and KH_2PO_4, 1.0 g/L. The rate of zinc in Ganoderma lucidum was up to 25.1% under the condition of the above-mentioned three kinds of nutrient components being added in the medium. [Conclusion] The key nutritional factors screened out with the method of Plackett-Burman Design was beneficial to high rate of zinc-richened Ganoderma lucidum culturing, which method was proved to be cost and effective. |
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Keywords: | Ganoderma lucidum Rate of zinc Medium Plackett-Burman Design |
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