Determination of Six Synthetic Pigments in Compound Wine by HPLC

The aim of this work was to establish a method that the 6 synthetic pigments in compound wine were determined by HPLC.The synthetic pigments in the compound wines were extracted by liquid-liquid extraction,separated by C18 symmetry chromatographic column,with 0.02 mol/L ammonium acetate and methanol as the mobile phase with gradient elution,the sample was detected by diode array detector.The test results showed that the artificial synthetic pigment in 0.20-20 μg/L range of concentration had a good linear relationship (r＞0.999 2),the detection limits were 0.000 16-0.001 04 g/kg,the recovery rates were 82.0％-97.0％,the relative standard deviation were 0.7％-3.5％.The method was simple,rapid,high sensitivity,good separation effect and can be widely used in the daily inspection work.