不同方法提取珊瑚总蛋白质的比较

以海南省近岸海域的鼻形鹿角珊瑚(Acropora nasuta)和箭排孔珊瑚(Seriatopora hystrix)2种硬珊瑚为研究对象,比较了TRIzol试剂提取法和BP-酚提取法在珊瑚蛋白质提取量、双向蛋白点数量和分布上的差异。结果表明,BP-酚抽提法的蛋白质得率相对较高。通过1-DE及2-DE电泳比较分析发现,2种方法获得的蛋白样品存在较大差异。BP-酚抽提法能够获得更多蛋白条带和点数,并且具有较少的拖尾现象。在低相对分子质量蛋白的提取方面,TRIzol试剂提取法具有优势;在高相对分子质量的提取方面,BP-酚抽法具有明显优势。研究结果有望为硬珊瑚的蛋白质组学研究奠定一定的基础。

Comparative Study of Different Methods for Extracting of Total Protein of Corals

Availability of high-quality protein is a prerequisite for proteomic research.Hard bones are major structures for corals,leading to certain interference in extracting of total protein of the corals.Therefore,it is necessary to choose an optimum extraction method for proteomic research in corals.Two species of corals Acropora nasuta and Seriatopora hystrix were extracted by using two extraction methods,TRIzol reagent extraction method and BP-phenol extraction method.The result showed that the protein yield of the extract by BP-phenol extraction was relatively higher.Analysis of the 1-DE and 2-DE electrophoresis showed that the protein samples of the extracts by the two extraction methods were relatively different.The BP-phenol extraction method produced more protein bands and spots with few tailings.The TRIzol reagent method had an advantage of extraction of low molecular weight while the BP-phenol extraction method had an obvious advantage of the extraction of large molecular weight.This result is expected to provide a certain foundation for proteomic studies of stony corals.