| 牛BBOX1基因3'UTR变异体的克隆及其多态性分析 |
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| 引用本文： | 宋雨霏,郭彦,辛友志,崔建伟,周国利. 牛BBOX1基因3'UTR变异体的克隆及其多态性分析[J]. 河南农业科学, 2017, 46(2). DOI: 10.15933/j.cnki.1004-3268.2017.02.025 |
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| 作者姓名： | 宋雨霏 郭彦 辛友志 崔建伟 周国利 |
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| 作者单位： | 聊城大学 生命科学学院,山东 聊城,252059 |
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| 基金项目： | 国家自然科学基金项目，山东省自然科学基金项目，聊城大学博士启动基金项目 |
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| 摘 要： | 为鉴定牛BBOX1基因选择性多聚腺苷酸化的不同变异体及其3'UTR的多态性,采用3'RACE的方法检测出BBOX1基因3个不同3'UTR全长的APA变异体,短APA变异体3'UTR长度为229 bp,长APA变异体3'UTR长度分别为664 bp和678 bp。利用SSCP与测序相结合的方法,在BBOX1基因的3'UTR中检测到10个多态性,其中7个为SNP,分别为c.12TC、c.100AG、c.241TC、c.480TC、c.557TC、c.606TC和c.650TC;3个为插入或缺失突变,分别为c.28_29ins C、c.434_435del GT和c.512_513ins TGC。SNP c.650TC定位在Poly(A)加尾信号PAS3中,使加尾信号序列AAUAAA突变成AACAAA,导致3'UTR长度为678 bp的变异体出现。
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| 关 键 词： | 牛 BBOX1基因 选择性多聚腺苷酸化 多态性 3'UTR |
Cloning of 3'UTR Isoforms and Polymorphisms in Bovine BBOX1 Gene |
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| SONG Yufei,GUO Yan,XIN Youzhi,CUI Jianwei,ZHOU Guoli. Cloning of 3'UTR Isoforms and Polymorphisms in Bovine BBOX1 Gene[J]. Journal of Henan Agricultural Sciences, 2017, 46(2). DOI: 10.15933/j.cnki.1004-3268.2017.02.025 |
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| Authors: | SONG Yufei GUO Yan XIN Youzhi CUI Jianwei ZHOU Guoli |
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| Abstract: | The objective of this study was to identify isoforms of 3'UTR and polymorphisms in 3'UTR regions of bovine BBOX1 gene. Three different alternative polyadenylation(APA) isoforms of 3'UTR in the BBOX1 gene were identified by 3′RACE. Their lengths were 229,664,678 bp, respectively. Ten polymorphisms loci were identified in 3'UTR of BBOX1 gene by single strand conformation polymorphism (SSCP) and sequencing. There were seven SNPs,including c. 12T>C,c. 100A>G,c. 241T>C,c. 480T>C, c. 557T>C, c. 606T >C, and c. 650T >C. Three were deletion/insertion mutations, including c. 2829insC,c. 434435delGT,and c. 512513insTGC. SNP c. 650T>C was located in polyadenylation signals sequence PAS3. Moreover,the mutation changed AAUAAA into AACAAA,resulting in presence of 678 bp isform of 3'UTR in bovine BBOX1 gene. |
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| Keywords: | cattle BBOX1 gene APA polymorphisms 3'UTR |
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