| 羔羊体外胚胎高效生产技术研究 |
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| 引用本文： | 佟桂芝,李信涛,宋斌,董正德,王伟霞,海龙,刘小辉,郭立宏,王嘉厚,王植朔.羔羊体外胚胎高效生产技术研究[J].农业科学与技术,2017,18(4). |
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| 作者姓名： | 佟桂芝 李信涛 宋斌 董正德 王伟霞 海龙 刘小辉 郭立宏 王嘉厚 王植朔 |
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| 作者单位： | 1. 黑龙江省畜牧研究所,黑龙江齐齐哈尔,161005;2. 吉林省农业科学研究院,吉林长春,130000 |
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| 基金项目： | 黑龙江省财政厅项目，齐齐哈尔市农业攻关项目(NYGG-201425).Supported by the Project of the Finance Department of Heilongjiang Province，the Key Program for Agriculture of Qiqihar City |
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| 摘 要： | 该研究旨在比较良种肉羊5周龄、13周龄羔羊激素处理后卵巢卵泡发育及体外胚胎发育的影响.通过FSH和孕马血清促性腺激素(PMSG)处理,比较卵巢上2~8 mm卵泡的数量及体外胚胎的发育情况.结果表明:5周龄羔羊卵巢发育的卵泡数和采集到的卵母细胞数(58.6±1.9、47.4±4.2)都高于13周龄羔羊卵泡数和卵母细胞数(14.4±4.1、11.6±1.7),且差异显著(P<0.05).5、13周龄羔羊卵母细胞受精后的卵裂率(60.0%±1.9%、61.6%±5.2%)及囊胚率(27.4%±2.1%、28.2%±3.7%),两者间差异不显著.
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| 关 键 词： | 激素 月龄 卵泡 羔羊 体外胚胎 |
Study on Efficient in Vitro Embryo Production Technology of Lambs |
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| Guizhi TONG,Xintao LI,Bin SONG,Zhengde DONG,Weixia WANG,Long HAI,Xiaohui LIU,Lihong GUO,Jiahou WANG,Zhishuo WANG.Study on Efficient in Vitro Embryo Production Technology of Lambs[J].Agricultural Science ＆ Technology,2017,18(4). |
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| Authors: | Guizhi TONG Xintao LI Bin SONG Zhengde DONG Weixia WANG Long HAI Xiaohui LIU Lihong GUO Jiahou WANG Zhishuo WANG |
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| Abstract: | The aim was to compare the effects of hormone treatments on the ovarian follicular development and in vitro embryo development between 5-week and 13-week old lambs. FSH and pregnant mare serum gonadotropin (PMSG) treatment were used to compare the number of 2-8 mm follicles in the ovary and the in vitro embryo development. The results showed that: the 5-week old lambs had larger follicular numbers and collected oocytes numbers (58.6±1.9, 47.4±4.2) than the 13-week old lambs (14.4±4.1, 11.6±1.7), and the difference was significant (P <0.05).However, there was no significant difference in the cleavage rate (60.0±1.9%, 61.6±5.2%) and blastocyst rate (27.4±2.1%, 28.2±3.7%) of the fertilized oocytes between the 5-week and 13-week old lambs. |
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| Keywords: | Hormone Month age Follicle Lamb In vitro embryo |
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